De Water R, Willems L N, Van Muijen G N, Franken C, Fransen J A, Dijkman J H, Kramps J A
Am Rev Respir Dis. 1986 May;133(5):882-90.
The present report describes the ultrastructural localization of bronchial antileukoprotease (ALP) in human central and peripheral airways by using polyclonal as well as monoclonal ALP-specific antibodies in a two-step gold-labeling procedure. In the serous cells of bronchial glands, ALP could be demonstrated in secretory granules. These granules, among which 4 phenotypes could be distinguished morphologically in ultrathin sections, showed the following labeling patterns: phenotype I, which had an electron lucent, fine granular content, and phenotype II, which was homogeneously electron dense, both showed gold label over their entire area. The granules expressing zonal differences in electron density (phenotype III) showed only label in their electron-dense cores and the electron-lucent granules (phenotype IV) showed a minimal labeling. Sometimes gold particles could be observed in the rough endoplasmic reticulum and nuclear envelope, suggesting that ALP is present in these cell organelles. In the bronchiolar epithelium, ALP could be localized only in the secretory granules of Clara cells and goblet cells. These findings indicate that ALP is also synthesized in bronchioli. To our knowledge this is the first time that a well-defined protein has been described that is produced and secreted by human Clara cells.
本报告描述了通过在两步金标法中使用多克隆及单克隆抗支气管抗白细胞蛋白酶（ALP）特异性抗体，对人中央气道和外周气道中支气管抗白细胞蛋白酶（ALP）进行超微结构定位的情况。在支气管腺的浆液细胞中，可在分泌颗粒中证实有ALP。这些颗粒在超薄切片中可在形态上区分出4种表型，呈现出以下标记模式：表型I，其内容物为电子透亮的细颗粒状；表型II，其电子密度均匀，二者在整个区域均显示有金标记。表现出电子密度区域差异的颗粒（表型III）仅在其电子致密核心显示有标记，而电子透亮颗粒（表型IV）显示的标记最少。有时在粗面内质网和核膜中可观察到金颗粒，提示这些细胞器中存在ALP。在细支气管上皮中，ALP仅定位于克拉拉细胞和杯状细胞的分泌颗粒中。这些发现表明细支气管中也合成ALP。据我们所知，这是首次描述由人克拉拉细胞产生并分泌的一种明确的蛋白质。
