Siddiqui Sazada
Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia.
Biology (Basel). 2024 Oct 9;13(10):805. doi: 10.3390/biology13100805.
The speedy growth of copper oxide nanoparticle (CuO NP) manufacturing due to their wide application in industries has caused concerns due to their increased discharge into the environment from both purposeful and accidental sources. Their presence at an elevated concentration in the environment can cause potential hazards to the plant kingdom, specifically to staple food crops. However, limited research is available to determine the consequences of CuO NPs. The present study aimed to assess the morphological and cytological changes induced by CuO NPs on L., a key staple food crop. Seeds of were exposed to various concentrations of CuO NPs (0, 25, 50, 75, 100, and 125 ppm) for 2 h, and their effects on seed germination (SG), radicle length (RL), cell proliferation kinetics (CPK), mitotic index (MI), cell death (CD), micronucleus frequency (MNF), and chromosomal aberration frequency (CAF) were studied. The results indicate a significant reduction in SG, RL, CPK, and MI and a significant dose-dependent increase in CD, MNF, and CAF. CuO NP treatment has led to abnormal meiotic cell division, increased incidence of micronucleus frequency, and chromosomal aberration frequency. Additionally, the CuO NP-treated groups showed an increase in the percentage of aberrant meiotic cells such as laggard (LG), double bridge (DB), stickiness (STC), clumped nuclei (CNi), precocious separation (PS), single bridge (SB), and secondary association (SA). CuO NP treatment led to reductions in SG as follows: 55% at 24 h, 60.10% at 48 h, and 65% at 72 h; reductions in RL as follows: 0.55 ± 0.021 cm at 24 h, 0.67 ± 0.01 cm at 48 h, and 0.99 ± 0.02 cm at 72 h; reductions in CPK as follows: 34.98% at prophase, 7.90% at metaphase, 3.5% at anaphase, and 0.97% at telophase. It also led to a 57.45% increase in CD, a 39.87% reduction in MI, and a 60.77% increase in MNF at a higher concentration of 125 ppm. The findings of this study clearly show that CuO NPs have a genotoxic effect on the food crop plant
由于氧化铜纳米颗粒（CuO NP）在工业中的广泛应用，其产量迅速增长，这引发了人们的担忧，因为无论是有意还是意外排放，它们进入环境的量都在增加。环境中其浓度升高可能会对植物界，特别是主粮作物造成潜在危害。然而，关于CuO NPs后果的研究有限。本研究旨在评估CuO NPs对一种关键主粮作物L.诱导的形态和细胞学变化。将L.的种子暴露于不同浓度的CuO NPs（0、25、50、75、100和125 ppm）中2小时，并研究其对种子萌发（SG）、胚根长度（RL）、细胞增殖动力学（CPK）、有丝分裂指数（MI）、细胞死亡（CD）、微核频率（MNF）和染色体畸变频率（CAF）的影响。结果表明，SG、RL、CPK和MI显著降低，而CD、MNF和CAF则呈显著的剂量依赖性增加。CuO NP处理导致减数分裂细胞异常分裂、微核频率和染色体畸变频率增加。此外，CuO NP处理组中落后染色体（LG）、双桥（DB）、粘连（STC）、核聚集（CNi）、早熟分离（PS）、单桥（SB）和二次联会（SA）等异常减数分裂细胞的百分比增加。CuO NP处理导致SG降低如下：24小时时降低55%，48小时时降低60.10%，72小时时降低65%；RL降低如下：24小时时为0.55±0.021厘米，48小时时为0.67±0.01厘米，72小时时为0.99±0.02厘米；CPK降低如下：前期降低34.98%，中期降低7.90%，后期降低3.5%，末期降低0.97%。在125 ppm的较高浓度下，它还导致CD增加57.45%，MI降低39.87%，MNF增加60.77%。本研究结果清楚地表明，CuO NPs对粮食作物植物具有遗传毒性作用
