IRCCS Humanitas Research Hospital, Department of Gynecology, Division of Gynecology and Reproductive Medicine, Fertility Centre, Rozzano, via Manzoni 56, 20089, Milan, Italy.
Humanitas University, Department of Biomedical Sciences, Pieve Emanuele, via Rita Levi Montalcini 4, 20090, Milan, Italy.
Reprod Sci. 2023 Sep;30(9):2866-2875. doi: 10.1007/s43032-023-01232-w. Epub 2023 Apr 17.
The purpose was to determine any difference in outcomes, primarily in terms of number of retrieved oocytes per procedure, between two different needles used for oocytes retrieval procedure in Assisted Reproductive Technologies: the single-lumen needle (SLN) versus the double-lumen needle (DLN) with follicle flushing after aspiration. This randomized controlled trial included oocyte retrieval (OR) cycles for IVF and ICSI performed in 18 to 42-year-old women between March 2019 and January 2021 at a tertiary-care Fertility Center. A total of 200 ORs were randomized, 100 in each group. The mean number of retrieved oocytes was not different between groups (10.2 ± 6.5 for DLNs vs. 10.7 ± 7.0 for SLNs, p = 0.810). No significant differences were observed also in terms of number of retrieved oocytes/punctured follicles (83.0% ± 27.0% vs. 81.0% ± 22.0%, p = 0.916), number of retrieved oocytes/follicles at trigger (78.0% ± 29.0% vs. 78.0% ± 27.0%, p = 0.881), number of mature oocytes (7.6 ± 5.3 vs. 8.0 ± 5.1, p = 0.519), and pregnancy rate (27% vs. 23%, p = 0.514). However, the time required to retrieve each oocyte was longer using the DLN (1.5 ± 1.3 vs. 1.1 ± 0.9 minutes, p = 0.002). The present study confirmed the new perspectives on the sole use of SLNs in terms of saving time, without affecting the number of retrieved oocytes. Trial registration number and date of registration NCT03611907; July 26, 2018.
目的是确定两种不同的取卵针（即单腔针[SLN]和抽吸后卵泡冲洗的双腔针[DLN]）在辅助生殖技术中的取卵过程中的结果是否存在差异，主要是在每个手术中取出的卵母细胞数量方面。这项随机对照试验纳入了 2019 年 3 月至 2021 年 1 月期间在一家三级保健生育中心接受体外受精和卵胞浆内单精子注射的 18 至 42 岁女性的取卵（OR）周期。共有 200 个 OR 被随机分为两组，每组 100 个。两组间取出的卵母细胞数量无显著差异（DLN 组为 10.2±6.5，SLN 组为 10.7±7.0，p=0.810）。在取出的卵母细胞/穿刺卵泡数（83.0%±27.0% vs. 81.0%±22.0%，p=0.916）、触发时取出的卵母细胞/卵泡数（78.0%±29.0% vs. 78.0%±27.0%，p=0.881）、成熟卵母细胞数（7.6±5.3 vs. 8.0±5.1，p=0.519）和妊娠率（27% vs. 23%，p=0.514）方面也无显著差异。然而，使用 DLN 时每个卵母细胞的取出时间更长（1.5±1.3 分钟 vs. 1.1±0.9 分钟，p=0.002）。本研究证实了在节省时间方面单独使用 SLN 的新观点，而不会影响取出的卵母细胞数量。试验注册号和注册日期 NCT03611907；2018 年 7 月 26 日。
