Department of Diagnostic Sciences and Oral Biology, Department of Pediatric Dentistry and Orthodontic Sciences, College of Dentistry, King Khalid University, Abha, Saudi Arabia.
Eur Rev Med Pharmacol Sci. 2023 Apr;27(7):2733-2738. doi: 10.26355/eurrev_202304_31900.
This study aimed to evaluate the effect of photodynamic therapy (PDT) on the salivary flow rate, secretory immunoglobulin A, and C-reactive protein levels in active smokers.
The present study is a prospective case-control study. Twenty active smokers were allocated to two groups randomly of ten participants each: the experimental group was irradiated while the control was exposed to sham irradiation by turning off the equipment. In the experimental group, methylene blue mediated PDT was applied both intra- and extra-orally over the major and minor salivary glands using a diode laser. 780 nm wavelength and 4 J/cm2 of energy were used to irradiate the 10 points of major salivary glands (6 for parotid and 2 for submandibular glands and 2 for sublingual glands). On the other hand, 660 nm was used to apply 10 J/cm2 of energy over the minor salivary glands at numerous points. The samples of the stimulated and unstimulated saliva were collected from both groups to assess the SFR. ELISA method was used to assess the level of salivary IgA levels, statistical analysis was done using a one-way ANOVA, and a p-value of <0.05 was considered significant.
The results showed a significant increment in salivary and secretory immunoglobulin A levels of subjects undergone photodynamic therapy. C-reactive protein levels were significantly decreased in subjects exposed to irradiation.
The present study concludes that photodynamic therapy significantly improves the salivary flow rate, secretory Immunoglobulin A, and oral health quality of life in smokers. The inflammatory salivary marker C-reactive protein, which is usually raised in smokers, is also reduced.
本研究旨在评估光动力疗法（PDT）对活跃吸烟者的唾液流量、分泌型免疫球蛋白 A 和 C 反应蛋白水平的影响。
本研究为前瞻性病例对照研究。将 20 名活跃吸烟者随机分为两组，每组 10 名参与者：实验组通过关闭设备进行假照射，而对照组则通过照射进行照射。在实验组中，通过二极管激光在口腔内外对大、小唾液腺进行亚甲蓝介导的 PDT。使用 780nm 波长和 4J/cm2 的能量照射大唾液腺的 10 个点（6 个用于腮腺，2 个用于颌下腺，2 个用于舌下腺）。另一方面，使用 660nm 波长在许多点上对小唾液腺施加 10J/cm2 的能量。从两组中收集刺激和非刺激唾液样本以评估 SFR。使用 ELISA 方法评估唾液 IgA 水平，使用单向方差分析进行统计分析，p 值<0.05 被认为具有统计学意义。
结果表明，接受光动力疗法的受试者的唾液和分泌型免疫球蛋白 A 水平显著增加。接受照射的受试者的 C 反应蛋白水平显著降低。
本研究得出结论，光动力疗法可显著提高吸烟者的唾液流量、分泌型免疫球蛋白 A 和口腔健康生活质量。通常在吸烟者中升高的炎症性唾液标志物 C 反应蛋白也降低了。
