基于抗体修饰金纳米颗粒的电化学生物传感器用于甲型流感病毒的快速灵敏分析。

Electrochemical biosensor based on antibody-modified Au nanoparticles for rapid and sensitive analysis of influenza A virus.

作者信息

Bao Qiwen, Li Gang, Yang Zhengchun, Liu Jun, Wang Hanjie, Pang Gaoju, Guo Qianjin, Wei Jun, Cheng Wenbo, Lin Ling

机构信息

School of Precision Instrument and Optoelectronic Engineering, the State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, 92 Weijin Road, Tianjin, 300072 China.

School of Electrical and Electronic Engineering, Tianjin Key Laboratory of Film Electronic & Communication Devices, Advanced Materials and Printed Electronics Center, Tianjin University of Technology, Tianjin, 300384 China.

出版信息

Ionics (Kiel). 2023;29(5):2021-2029. doi: 10.1007/s11581-023-04944-w. Epub 2023 Mar 9.

UNLABELLED

To cope with the easy transmissibility of the avian influenza A virus subtype H1N1, a biosensor was developed for rapid and highly sensitive electrochemical immunoassay. Based on the principle of specific binding between antibody and virus molecules, the active molecule-antibody-adapter structure was formed on the surface of an Au NP substrate electrode; it included a highly specific surface area and good electrochemical activity for selective amplification detection of the H1N1 virus. The electrochemical test results showed that the BSA/H1N1 Ab/Glu/Cys/Au NPs/CP electrode was used for the electrochemical detection of the H1N1 virus with a sensitivity of 92.1 µA (pg/mL) cm, LOD of 0.25 pg/ml, linear ranges of 0.25-5 pg/mL, and linearity of ( = 0.9846). A convenient H1N1 antibody-based electrochemical electrode for the molecular detection of the H1N1 virus will be of great use in the field of epidemic prevention and raw poultry protection.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s11581-023-04944-w.

未标注

为应对甲型H1N1禽流感病毒的易传播性，开发了一种用于快速、高灵敏度电化学免疫分析的生物传感器。基于抗体与病毒分子之间特异性结合的原理，在金纳米颗粒（Au NP）基底电极表面形成了活性分子-抗体-适配体结构；它具有高比表面积和良好的电化学活性，用于对H1N1病毒进行选择性放大检测。电化学测试结果表明，BSA/H1N1 Ab/Glu/Cys/Au NPs/CP电极用于H1N1病毒的电化学检测，灵敏度为92.1 μA/(pg/mL)·cm，检测限为0.25 pg/ml，线性范围为0.25 - 5 pg/mL，线性度为（ = 0.9846）。一种基于H1N1抗体的便捷电化学电极用于H1N1病毒的分子检测，将在防疫和生禽保护领域有很大用途。