Shakhlamov V A, Barkhina T G, Grun'ko V A
Arkh Anat Gistol Embriol. 1986 Mar;90(3):55-60.
Ultrastructure of the primary culture of epitheliocytes in the small and large intestine of 20-22-week-old human fetuses has been investigated, normal and 1, 3, 6, 12 and 24 h after administration of cholera toxin (choleragen) into the cell culture. The culture studied is mainly presented by absorbtive epitheliocytes, goblet, endocrinic and Paneth's cells, that preserve to a certain degree their differented structure. The most pronounced ultrastructural changes of the intestinal epitheliocytes in the primary cell culture, resembling those, that are previously noted in the epitheliocytes of the small and large intestine under influence of cholera toxin in various experimental animals in vivo, are revealed 3-6 h after administration of cholera toxin into the primary culture of the small intestine epitheliocytes and 6-12 h--into the large intestine epitheliocytes. The intestinal epitheliocytes of the human fetuses in the cell culture are sensitive to the action of cholera toxin and present a suitable model for studying the action mechanism of the toxin on the intestinal epithelium.
对20 - 22周龄人类胎儿小肠和大肠上皮细胞原代培养物的超微结构进行了研究，研究了细胞培养物在正常情况下以及在向其中加入霍乱毒素（霍乱原）后1、3、6、12和24小时的情况。所研究的培养物主要由吸收性上皮细胞、杯状细胞、内分泌细胞和潘氏细胞组成，这些细胞在一定程度上保留了它们的分化结构。在小肠上皮细胞原代培养物中加入霍乱毒素后3 - 6小时，以及在大肠上皮细胞中加入霍乱毒素后6 - 12小时，发现原代细胞培养物中肠道上皮细胞最明显的超微结构变化，类似于先前在各种实验动物体内霍乱毒素影响下小肠和大肠上皮细胞中所观察到的变化。细胞培养物中人类胎儿的肠道上皮细胞对霍乱毒素的作用敏感，是研究该毒素对肠道上皮作用机制的合适模型。
