蛋白 Rpr2 和 Pop3 可提高酵母 RNase P 的活性和热稳定性。

Department of Biochemistry and Molecular Biology, Center for RNA Biology, Pennsylvania State University, University Park, PA, USA.

RNA Biol. 2023 Jan;20(1):149-153. doi: 10.1080/15476286.2023.2201110.

RNA-based enzyme RNase P is a ribonucleoprotein complex responsible primarily for 5'-maturation of tRNAs. RNase P comprises a catalytic RNA component and nine proteins. The assembly and maturation of RNase P involves an abundant and catalytically active precursor form, which includes all components except for proteins Rpr2 and Pop3. Rpr2 and Pop3 are essential proteins, but their roles in RNase P were not clear. Here we use a step-wise in vitro assembly of yeast RNase P to show that the addition of proteins Rpr2 and Pop3 increases the activity and thermal stability of the RNase P complex, similar to the effects previously observed for archaeal RNases P.

基于 RNA 的酶 RNase P 是一种核糖核蛋白复合物，主要负责 tRNA 的 5'-成熟。RNase P 由一个催化 RNA 成分和九个蛋白质组成。RNase P 的组装和成熟涉及丰富且具有催化活性的前体形式，其中包含除了蛋白质 Rpr2 和 Pop3 以外的所有成分。Rpr2 和 Pop3 是必需蛋白质，但它们在 RNase P 中的作用尚不清楚。在这里，我们使用酵母 RNase P 的逐步体外组装来表明，添加蛋白质 Rpr2 和 Pop3 会提高 RNase P 复合物的活性和热稳定性，这类似于先前观察到的古菌 RNase P 的效果。