Wortzel Inbal, Porat Ziv
Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.
Flow Cytometry Unit, Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.
Methods Mol Biol. 2023;2635:173-184. doi: 10.1007/978-1-0716-3020-4_10.
Unlike the common conception of the Golgi apparatus as a static organelle, it is, in fact, a dynamic structure, as well as a sensitive sensor for the cellular status. In response to various stimuli, the intact Golgi structure undergoes fragmentation. This fragmentation can yield either partial fragmentation, resulting in several separated chunks, or complete vesiculation of the organelle. These distinct morphologies form the basis of several methods for the quantification of the Golgi status. In this chapter, we describe our imaging flow cytometry-based method for quantifying changes in the Golgi architecture. This method has all the benefits of imaging flow cytometry-namely, it is rapid, high-throughput, and robust-while affording easy implementation and analysis capabilities.
与将高尔基体视为静态细胞器的普遍观念不同，实际上它是一种动态结构，也是细胞状态的敏感传感器。响应各种刺激时，完整的高尔基体结构会发生碎片化。这种碎片化可能导致部分碎片化，形成几个分离的碎片，或者导致该细胞器完全形成囊泡。这些不同的形态构成了几种高尔基体状态定量方法的基础。在本章中，我们描述了基于成像流式细胞术的高尔基体结构变化定量方法。该方法具有成像流式细胞术的所有优点——即快速、高通量且稳健——同时具备易于实施和分析的能力。
