Department of Biological Regulation, Weizmann Institute of Science, Rehovot 7610001, Israel.
Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot 7610001, Israel.
STAR Protoc. 2022 Apr 5;3(2):101278. doi: 10.1016/j.xpro.2022.101278. eCollection 2022 Jun 17.
The Golgi apparatus is subjected to fragmentation under several cellular processes such as mitosis. Here we describe two complementary approaches to analyze different Golgi morphological changes during its mitotic fragmentation, using classical immunofluorescence and imaging flow cytometry. Although fluorescent microscopy provides information on the exact Golgi architecture in distinct cells, the imaging flow cytometry combines the morphological data with the high-throughput quantification of flow cytometry. Taken together, both approaches provide robust and significant unbiased data analysis. For complete details on the use and execution of this protocol, please refer to Wortzel et al. (2021).
高尔基器在有丝分裂等几种细胞过程中会发生碎裂。在这里，我们描述了两种互补的方法，使用经典免疫荧光和成像流式细胞术来分析有丝分裂过程中高尔基器形态的不同变化。虽然荧光显微镜可以提供关于不同细胞中高尔基器确切结构的信息，但成像流式细胞术将形态学数据与流式细胞术的高通量定量相结合。总之，这两种方法都提供了强大而有意义的无偏数据分析。有关该方案使用和执行的完整详细信息，请参见 Wortzel 等人（2021 年）。
