Departamento de Ciências Químicas e Biológicas, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brasil.
Hemocentro de Botucatu, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP, Brasil.
Braz J Med Biol Res. 2023 Apr 14;56:e12659. doi: 10.1590/1414-431X2023e12659. eCollection 2023.
Dendritic cells (DCs) are antigen-presenting cells that drive the differentiation of T CD4+ cells into different profiles according to the nature of the antigen or immunomodulator. Propolis is a resinous product made by bees that has numerous pharmacological properties, including an immunomodulatory action. To assess whether propolis can modulate the activation of CD4+ T cells by stimulating DCs with heat-labile enterotoxin B subunit (EtxB) or lipopolysaccharide (LPS), we aimed to elucidate the mechanisms affected by propolis in the differential activation of T lymphocytes. Cell viability, lymphocyte proliferation, gene expression (GATA-3 and RORc), and cytokine production (interleukin (IL)-4 and IL-17A) were analyzed. Propolis, EtxB, and LPS induced a higher lymphoproliferation compared with the control. Propolis induced GATA-3 expression and, in combination with EtxB, maintained the baseline levels. Propolis alone or in combination with LPS inhibited RORc expression. EtxB alone and in combination with propolis increased IL-4 production. Propolis in combination with LPS prevented LPS-induced IL-17A production. These results opened perspectives for the study of biological events that may be favored by propolis by promoting Th2 activation or helping in the treatment of inflammatory conditions mediated by Th17 cells.
树突状细胞 (DCs) 是抗原呈递细胞，根据抗原或免疫调节剂的性质，将 T CD4+ 细胞分化为不同的表型。蜂胶是蜜蜂产生的一种树脂状产物，具有多种药理学特性，包括免疫调节作用。为了评估蜂胶是否可以通过用不耐热肠毒素 B 亚单位 (EtxB) 或脂多糖 (LPS) 刺激 DC 来调节 CD4+T 细胞的激活，我们旨在阐明蜂胶在 T 淋巴细胞的差异激活中影响的机制。分析了细胞活力、淋巴细胞增殖、基因表达（GATA-3 和 RORc）和细胞因子产生（白细胞介素 (IL)-4 和 IL-17A）。与对照组相比，蜂胶、EtxB 和 LPS 诱导更高的淋巴细胞增殖。蜂胶诱导 GATA-3 表达，并与 EtxB 一起维持基线水平。单独的蜂胶或与 LPS 联合抑制 RORc 表达。单独的 EtxB 以及与蜂胶联合增加了 IL-4 的产生。蜂胶与 LPS 联合防止了 LPS 诱导的 IL-17A 产生。这些结果为研究可能通过促进 Th2 激活或有助于治疗由 Th17 细胞介导的炎症状态而受到蜂胶促进的生物学事件开辟了前景。
