State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300350, People's Republic of China.
School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.
Carbohydr Polym. 2020 Oct 1;245:116469. doi: 10.1016/j.carbpol.2020.116469. Epub 2020 May 26.
A novel polysaccharide (ILP50-2) was extracted, isolated and purified from the leaves of Ilex latifolia Thunb. Its structure was characterized as a repeating unit consisting of α-L-Araf-(1→, →3)-α-L-Araf-(1→, →5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, β-D-Galp-(1→, →4)-β-D-Galp-(1→, →4)-β-D-Glcp-(1→, →6)-α-D-Manp-(1→, and →3,6)-α-D-Galp-(1→. The absolute molecular weight of ILP50-2 was 1.49 × 10 g/mol, which adapted a compact coil conformation in 0.1 M NaCl solution with R of 25.4 nm. Furthermore, ILP50-2 exhibited immunoregulatory activity, mainly through enhancing the phagocytosis ability of macrophages and prompting the release of nitric oxide (NO) and cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). Simultaneously, ILP50-2 was found to significantly increase the release of ROS and NO in zebrafish embryos, showing immunoregulatory effects in vivo.
从冬青叶中提取、分离和纯化出一种新型多糖（ILP50-2）。其结构特征为重复单元，由α-L-Araf-(1→, →3)-α-L-Araf-(1→, →5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, β-D-Galp-(1→, →4)-β-D-Galp-(1→, →4)-β-D-Glcp-(1→, →6)-α-D-Manp-(1→, 和 →3,6)-α-D-Galp-(1→组成。ILP50-2 的分子量为 1.49 × 10 g/mol，在 0.1 M NaCl 溶液中采用紧凑的线圈构象，回旋半径为 25.4nm。此外，ILP50-2 表现出免疫调节活性，主要通过增强巨噬细胞的吞噬能力，并促使一氧化氮（NO）和细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的释放。同时，ILP50-2 被发现能显著增加斑马鱼胚胎中 ROS 和 NO 的释放，在体内表现出免疫调节作用。
