评价 3-甲基苯丙胺滥用患者中 microRNA let-7b-3p 的表达水平。
Evaluation of microRNA let-7b-3p expression levels in methamphetamine abuse.
机构信息
Cukurova University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology - Adana, Turkey.
University of Health Sciences, Hamidiye Institute of Health Sciences, Department of Molecular Biology and Genetics - İstanbul, Turkey.
出版信息
Rev Assoc Med Bras (1992). 2023 Apr 17;69(4):e20221391. doi: 10.1590/1806-9282.20221391. eCollection 2023.
OBJECTIVE
In this study, we aimed to identify a microRNA expression signature that could be used to distinguish methamphetamine from control samples. We also utilized the existing bioinformatics tools in order to predict the candidate microRNAs that could play potential key roles in regulating drug addiction-related genes.
METHODS
Methamphetamine samples from 21 ventral tegmental area and 21 nucleus accumbens samples and their control regions were obtained from the Council of Forensic Medicine (Istanbul). Quantitative analysis of let-7b-3p was studied using quantitative reverse transcription PCR. Statistical analysis was carried out using Student's t-test. The receiver operating characteristic curves were plotted with Statistical Package for the Social Sciences (SPSS 20.0).
RESULTS
Our quantitative reverse transcription PCR results revealed that let-7b-3p was significantly overexpressed in brain tissues of the methamphetamine-user group. Let-7b-3p had significant power to discriminate methamphetamine from control samples in the ventral tegmental area (AUC; 0.922) and nucleus accumbens (AUC; 0.899) regions.
CONCLUSION
We have shown for the first time in the literature the differential expression of let-7b-3p in samples from methamphetamine-addicted individuals. We suggest that let-7b-3p could be a powerful marker for the diagnosis of methamphetamine addiction. Our results showed that differentially expressed let-7b-3p in methamphetamine users could be used as a diagnostic and therapeutic marker.
目的
在这项研究中，我们旨在确定一种可以用于区分冰毒和对照样本的 microRNA 表达特征。我们还利用现有的生物信息学工具来预测可能在调节与药物成瘾相关基因方面发挥潜在关键作用的候选 microRNAs。
方法
从法医委员会（伊斯坦布尔）获得了 21 个腹侧被盖区和 21 个伏隔核样本及其对照区域的冰毒样本。使用定量逆转录 PCR 研究 let-7b-3p 的定量分析。使用 Student's t-test 进行统计分析。使用社会科学统计软件包（SPSS 20.0）绘制受试者工作特征曲线。
结果
我们的定量逆转录 PCR 结果显示，冰毒使用者的脑组织中 let-7b-3p 表达显著上调。Let-7b-3p 在腹侧被盖区（AUC；0.922）和伏隔核（AUC；0.899）区域具有区分冰毒和对照样本的显著能力。
结论
我们首次在文献中显示了冰毒成瘾者样本中 let-7b-3p 的差异表达。我们认为 let-7b-3p 可能是冰毒成瘾诊断的有力标志物。我们的结果表明，冰毒使用者中差异表达的 let-7b-3p 可用作诊断和治疗标志物。
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