胰高血糖素和正辛酸对培养肝细胞中丙酮酸脱氢酶激酶活性的调节作用。
Modulation of pyruvate dehydrogenase kinase activity in cultured hepatocytes by glucagon and n-octanoate.
作者信息
Fatania H R, Vary T C, Randle P J
出版信息
Biochem J. 1986 Feb 15;234(1):233-6. doi: 10.1042/bj2340233.
Abstract
The activity of pyruvate dehydrogenase kinase in extracts of mitochondria from rat hepatocytes cultured for 21 h in medium 199 was increased 2.5-fold by the presence of 55 nM-glucagon and 1 mM-sodium n-octanoate in the culture medium. The change was comparable with that induced in vivo by 48 h starvation. The potential contribution of branched-chain complex to estimates of PDH-complex activity in rat liver mitochondria has been defined.
摘要
在199培养基中培养21小时的大鼠肝细胞线粒体提取物中，培养基中55 nM胰高血糖素和1 mM正辛酸钠的存在使丙酮酸脱氢酶激酶的活性增加了2.5倍。这种变化与48小时饥饿在体内诱导的变化相当。已确定支链复合物对大鼠肝脏线粒体中PDH复合物活性估计值的潜在贡献。
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