表面预反应玻璃离子（S-PRG）填料洗脱液对混合微生物生物膜的抗菌作用。

Antibacterial effects of surface pre-reacted glass-ionomer (S-PRG) filler eluate on polymicrobial biofilms.

作者信息

Tomiyama Kiyoshi, Ishizawa Masato, Watanabe Kiyoko, Kawata Akira, Hamada Nobushiro, Mukai Yoshiharu

机构信息

Department of Restorative Dentistry, Kanagawa Dental University, Yokosuka, Kanagawa, Japan.

Department of Oral Microbiology, Kanagawa Dental University, Yokosuka, Kanagawa, Japan.

出版信息

Am J Dent. 2023 Apr;36(2):91-94.

PMID:37076299

Abstract

PURPOSE

To analyze the effects of surface pre-reacted glass-ionomer (S-PRG) filler eluate on polymicrobial biofilm metabolism and live bacterial count.

METHODS

Biofilm was formed using glass disks 12 mm in diameter and 150 µm in thickness. Stimulated saliva was diluted 50-fold with buffered McBain 2005 and cultured in anaerobic conditions at 37°C for 24 hours in anaerobic conditions (10% CO₂, 10% H₂, 80% N₂) to form the biofilm on the glass disks. Following this, biofilms were treated with (1) sterilized deionized water (control), (2) 0.2% chlorhexidine digluconate (0.2CX), (3) S-PRG eluate diluted to 10% (10% S-PRG)，(4) 20% S-PRG，(5) 40% S-PRG，(6) 80% S-PRG，and (7) S-PRG for 15 minutes (n= 10 per group), and samples were subdivided into two groups for measuring live bacterial count immediately after treatment and after 48 hours of culturing after treatment. The pH of the spent medium collected at the time of culture medium exchange was tested.

RESULTS

Immediately after treatment, the live bacterial count of samples treated with drug solutions was significantly lower than the control (8.2 × 10⁸), and the counts of samples treated with 0.2CX (1.3 × 10⁷) and S-PRG (1.4 × 10⁷) were significantly lower than those treated with diluted S-PRG (4.4 × 10⁷-1.4 x 10⁸). When the medium was measured again after culturing for 48 hours, growth was continually inhibited in all treatment groups and the bacterial count of samples treated with S-PRG (9.2 x 10⁷) was significantly lower than that of samples treated with 0.2CX (1.8 × 10⁸). The pH of spent medium immediately after treatment was significantly higher in groups treated with drug solutions (5.5-6.8) than the controls (4.2), and it was highest in the S-PRG-treated group (6.8). Thereafter, when culturing was continued for 48 hours, the pH of all treated groups decreased; however, the pH of the S-PRG-treated group was significantly higher than groups treated with other drug solutions.

CLINICAL SIGNIFICANCE

Surface pre-reacted glass-ionomer (S-PRG) filler eluate not only reduced the live bacterial count of polymicrobial biofilm, but also continuously inhibited the lowering of pH.

摘要

目的

分析表面预反应玻璃离子（S-PRG）填料洗脱液对多微生物生物膜代谢及活菌计数的影响。

方法

使用直径12毫米、厚度150微米的玻璃盘形成生物膜。将刺激唾液用缓冲的McBain 2005稀释50倍，并在37°C的厌氧条件下（10% CO₂、10% H₂、80% N₂）培养24小时，以在玻璃盘上形成生物膜。此后，生物膜用以下物质处理：（1）灭菌去离子水（对照），（2）0.2%葡萄糖酸洗必泰（0.2CX），（3）稀释至10%的S-PRG洗脱液（10% S-PRG），（4）20% S-PRG，（5）40% S-PRG，（6）80% S-PRG，以及（7）S-PRG处理15分钟（每组n = 10），样本被分成两组，分别用于在处理后立即以及处理后培养48小时后测量活菌计数。测试在更换培养基时收集的用过培养基的pH值。

结果

处理后立即测量，用药物溶液处理的样本的活菌计数显著低于对照组（8.2×10⁸），用0.2CX（1.3×10⁷）和S-PRG（1.4×10⁷）处理的样本的计数显著低于用稀释S-PRG处理的样本（4.4×10⁷ - 1.4×10⁸）。在培养48小时后再次测量培养基时，所有处理组的生长均持续受到抑制，用S-PRG处理的样本的细菌计数（9.2×10⁷）显著低于用0.2CX处理的样本（1.8×10⁸）。处理后立即测量，用药物溶液处理的组的用过培养基的pH值（5.5 - 6.8）显著高于对照组（4.2），且在S-PRG处理组中最高（6.8）。此后，当继续培养48小时时，所有处理组的pH值均下降；然而，S-PRG处理组的pH值显著高于用其他药物溶液处理的组。