Department of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Department of Oral & Maxillofacial Oncology and Surgery, Osaka University Graduate School of Dentistry, Suita 565-0871, Japan.
Int J Mol Sci. 2024 Sep 2;25(17):9541. doi: 10.3390/ijms25179541.
The surface pre-reacted glass ionomer (S-PRG) filler is a type of bioactive functional glass that releases six different ions. This study examined the effects of the S-PRG filler eluate on in the presence of sucrose. In a solution containing , the concentrations of BO, Al, Sr, and F were significantly higher in the presence of the S-PRG filler eluate than in its absence ( < 0.001). The concentrations of these ions further increased in the presence of sucrose. Additionally, the S-PRG filler eluate significantly reduced glucan formation by ( < 0.001) and significantly increased the pH of the bacterial suspension ( < 0.001). Bioinformatic analyses revealed that the S-PRG filler eluate downregulated genes involved in purine biosynthesis (purC, purF, purL, purM, and purN) and upregulated genes involved in osmotic pressure (opuAa and opuAb). At a low pH (5.0), the S-PRG filler eluate completely inhibited the growth of in the presence of sucrose and significantly increased the osmotic pressure of the bacterial suspension compared with the control ( < 0.001). These findings suggest that ions released from the S-PRG filler induce gene expression changes and exert an inhibitory effect on in the presence of sucrose.
表面预反应玻璃离子（S-PRG）填料是一种具有生物活性的功能性玻璃，可释放六种不同的离子。本研究考察了 S-PRG 填料浸提液在存在蔗糖的情况下对 的影响。在含有 的溶液中，与不存在 S-PRG 填料浸提液相比，存在 S-PRG 填料浸提液时 BO、Al、Sr 和 F 的浓度显著升高（<0.001）。在存在蔗糖的情况下，这些离子的浓度进一步增加。此外，S-PRG 填料浸提液显著降低了 （<0.001）形成的葡聚糖，并显著增加了细菌悬浮液的 pH 值（<0.001）。生物信息学分析表明，S-PRG 填料浸提液下调了参与嘌呤生物合成的基因（purC、purF、purL、purM 和 purN），并上调了参与渗透压的基因（opuAa 和 opuAb）。在低 pH 值（5.0）下，S-PRG 填料浸提液在存在蔗糖的情况下完全抑制了 的生长，并与对照相比显著增加了细菌悬浮液的渗透压（<0.001）。这些发现表明，S-PRG 填料释放的离子诱导基因表达变化，并在存在蔗糖的情况下对 发挥抑制作用。
