Inhibitory Effects of Surface Pre-Reacted Glass Ionomer Filler Eluate on in the Presence of Sucrose.

The surface pre-reacted glass ionomer (S-PRG) filler is a type of bioactive functional glass that releases six different ions. This study examined the effects of the S-PRG filler eluate on in the presence of sucrose. In a solution containing , the concentrations of BO, Al, Sr, and F were significantly higher in the presence of the S-PRG filler eluate than in its absence ( < 0.001). The concentrations of these ions further increased in the presence of sucrose. Additionally, the S-PRG filler eluate significantly reduced glucan formation by ( < 0.001) and significantly increased the pH of the bacterial suspension ( < 0.001). Bioinformatic analyses revealed that the S-PRG filler eluate downregulated genes involved in purine biosynthesis (purC, purF, purL, purM, and purN) and upregulated genes involved in osmotic pressure (opuAa and opuAb). At a low pH (5.0), the S-PRG filler eluate completely inhibited the growth of in the presence of sucrose and significantly increased the osmotic pressure of the bacterial suspension compared with the control ( < 0.001). These findings suggest that ions released from the S-PRG filler induce gene expression changes and exert an inhibitory effect on in the presence of sucrose.