de Lima Gustavo Castro, de Cassia Orlando Sardi Janaina, de Figueiredo Luciene Cristina, Bueno-Silva Bruno, Fonseca Micaele Araújo, Dudu-Silva Gustavo, de Carvalho Felícia Priscila Pozzetti, Rodrigues José Augusto
School of Dentistry, Guarulhos University UNIVERITAS/UNG, Praça Tereza Cristina, 88 - Centro, Guarulhos, SP, 07023-070, Brazil.
Department of Biosciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, SP, Brazil.
Odontology. 2025 Jul 17. doi: 10.1007/s10266-025-01153-x.
This study evaluated the antibacterial activity of three S-PRG composite resins on the microbial profile of subgingival multispecies biofilm. A 96-well cell plate was employed to cultivate a 39-species biofilm associated with periodontitis over 7 days. Cylindrical specimens with 2 mm high and 2 mm diameters were prepared of each bioactive tested group (Beautifil II, Beautifil LS, Beautifil Bulk-Shofu) and for a control non-bioactive group (Z350 XT-3M ESPE). Furthermore, these specimens were immersed in the well plate to allow biofilm formation, and after growing for 7 days, the formed biofilm was removed and composition evaluated. The metabolic biofilm activity was evaluated by colorimetric assay, and the microbial profile evaluated by DNA-DNA hybridization. Statistical analysis was performed by Kruskal-Wallis followed by Mann-Whitney tests to evaluate each bacteria counts and ANOVA followed by T test to evaluate the proportion among complexes groups. A statistically significant reduction was observed on the counts of biofilm bacterial specie: Streptococcus gordoni, Streptococcus mitis, Streptococcus sanguinis, Campylobacter rectus, Campylobacter showae, Fusobacterium periodonticum, Tannerella forsythia, Neisseria mucosa, Selenomonas noxia and Streptococcus mutans for one or more bioactive composite resin compared to Z350XT (p ≤ 0.05). There was a significant reduction in the proportion of Yellow, Orange bacterial complexes, and others oral bacteria tested between the control and bioactive groups. Bioactive groups showed a statistically significant reduction in total counts of bacteria. The incorporation of S-PRG glass into the bioactive composite resin reduced periodontopathogenic biofilm growth.
本研究评估了三种含锶、镧的玻璃离子复合体（S-PRG）复合树脂对龈下多种细菌生物膜微生物构成的抗菌活性。采用96孔细胞培养板，在7天内培养与牙周炎相关的39种细菌生物膜。为每个生物活性测试组（Beautifil II、Beautifil LS、Beautifil Bulk-Shofu）以及一个对照非生物活性组（Z350 XT-3M ESPE）制备高2 mm、直径2 mm的圆柱形标本。此外，将这些标本浸入孔板中以形成生物膜，培养7天后，去除形成的生物膜并评估其组成。通过比色法评估生物膜的代谢活性，通过DNA-DNA杂交评估微生物构成。采用Kruskal-Wallis检验，随后进行Mann-Whitney检验进行统计分析，以评估每种细菌的数量；采用方差分析，随后进行t检验评估各复合体组之间的比例。与Z350XT相比，一种或多种生物活性复合树脂使生物膜细菌种类（戈登链球菌、缓症链球菌、血链球菌、直肠弯曲菌、昭和弯曲菌、牙周梭杆菌、福赛坦氏菌、黏膜奈瑟菌、有害月形单胞菌和变形链球菌）的数量有统计学意义的减少（p≤0.05）。对照和生物活性组之间，黄色、橙色细菌复合体以及其他测试口腔细菌的比例有显著降低。生物活性组的细菌总数有统计学意义的减少。将S-PRG玻璃加入生物活性复合树脂中可减少牙周致病生物膜的生长。
