窖蛋白-1 通过调节热休克蛋白 70 的表达促进慢性阻塞性肺疾病中 Th17/Treg 的失衡。
Caveolin-1 Promotes the Imbalance of Th17/Treg in Chronic Obstructive Pulmonary Disease by Regulating Hsp70 Expression.
机构信息
Department of Oncology, Affiliated Qingdao Central Hospital of Qingdao University, Qingdao Cancer Hospital, Qingdao, Shandong, 266042, People's Republic of China.
Department of Pathology, No. 971 Hospital of People's Liberation Army Navy, Qingdao, 266071, People's Republic of China.
出版信息
Int J Chron Obstruct Pulmon Dis. 2023 Apr 12;18:565-574. doi: 10.2147/COPD.S398780. eCollection 2023.
OBJECTIVE
To investigate whether the expression of Hsp70 is associated with Cav-1 in promoting the imbalance of Th17/Treg cells in COPD.
METHODS
The plasma Cav-1, Hsp70 expression were quantified by enzyme-linked immunosorbent assay (ELISA). The frequencies of circulating Th17, Treg cells and Th17/Treg ratio were determined by flow cytometry. Peripheral blood mononuclear cells (PBMCs) from subjects were transfected with Cav-1 or control plasmids and Hsp70 plasmid.
RESULTS
We found that Cav-1 expression was lower but the levels of Hsp70 and Th17 cells were higher in COPD than in healthy control (HC). Hsp70 expressions were positively correlated with Cav-1 levels, Th17 cells, and Th17/Treg ratio in COPD but not in HC. Cav-1 over-expression resulted in an increase in Hsp70 and Th17 levels. Suppressing Hsp70 expressing by small interfering RNA (siRNA), the decline of Th17 frequency was observed in Cav-1-overexpressed PBMCs.
CONCLUSION
Collectively, our results illuminate that Cav-1 contributes to the imbalance of Th17/Treg through potentially regulating Hsp70 expression.
目的
探讨热休克蛋白 70（Hsp70）的表达是否与 Cav-1 共同作用，促进 COPD 中 Th17/Treg 细胞失衡。
方法
通过酶联免疫吸附试验（ELISA）定量检测血浆 Cav-1、Hsp70 的表达。采用流式细胞术检测循环 Th17、Treg 细胞的频率及 Th17/Treg 比值。用 Cav-1 或对照质粒和 Hsp70 质粒转染受试者的外周血单个核细胞（PBMC）。
结果
我们发现 COPD 患者的 Cav-1 表达较低，但 Hsp70 和 Th17 细胞水平较高。COPD 患者中 Hsp70 的表达与 Cav-1 水平、Th17 细胞和 Th17/Treg 比值呈正相关，但在 HC 中无此相关性。Cav-1 过表达导致 Hsp70 和 Th17 水平升高。用小干扰 RNA（siRNA）抑制 Hsp70 表达，在 Cav-1 过表达的 PBMC 中观察到 Th17 频率下降。
结论
综上所述，我们的研究结果表明，Cav-1 通过潜在调节 Hsp70 表达，促进 Th17/Treg 失衡。
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