Hamada H, Asakura T, Matsuda K, Wechsler W, Rathmer K, Krajewski S
No To Shinkei. 1986 Mar;38(3):281-7.
As a rapid screening method for testing chemotherapeutic agents against human tumor explants, renal subcapsular assay has been established by Bogden et al (1978). The authors investigated the effectiveness of the technique of Bogden for rat glioma. Preselected 1 mm3 fragments of viable tumor tissue of rat (CDF) were implanted under the renal capsule of normal immunocompetent rats. Then initial and final tumor sizes (formula; see text) were measured in situ with an oculometer inserted into a surgical microscope, and evaluated the changes of tumor sizes. The experiments were divided into three groups. The first group was that tumor pieces of rat (CDF) were implanted under the renal capsule of the syngenetic rats (CDF). Non-irradiated and preirradiated (400 rad) heterogenetic rats (Wistar) were used as the second and third groups. The explanted tumor pieces grew rapidly in the first group (syngenetic rats). On the other hand, poor growth of the second group (non-irradiated heterogenetic rats) was seen within 7 days after implantation, and rejected completely until 15 days after implantation. Explanted tumor pieces in the third group (pre-irradiated heterogenetic rats), however, grew enough big, though the change of tumor sizes was less than that of the first group. Histological examination was also performed. In the first and third group, proliferation of glioma cells was remarkable under the renal capsule, and neovascularization in the explanted tumor tissue was provided from the capsule, first, and then parenchym of the kidney. Even necrosis and hemorrhage were seen in the tumor tissue 15 days after implantation.(ABSTRACT TRUNCATED AT 250 WORDS)
作为一种针对人类肿瘤外植体测试化疗药物的快速筛选方法，肾被膜下测定法已由博登等人（1978年）建立。作者研究了博登技术对大鼠神经胶质瘤的有效性。预先选择1立方毫米的大鼠（CDF）活肿瘤组织碎片，植入正常具有免疫活性的大鼠肾被膜下。然后用插入手术显微镜的目镜测微计在原位测量初始和最终肿瘤大小（公式；见正文），并评估肿瘤大小的变化。实验分为三组。第一组是将大鼠（CDF）的肿瘤块植入同基因大鼠（CDF）的肾被膜下。未照射和预先照射（400拉德）的异基因大鼠（Wistar）分别用作第二组和第三组。植入的肿瘤块在第一组（同基因大鼠）中生长迅速。另一方面，第二组（未照射的异基因大鼠）植入后7天内生长不良，到植入后15天完全被排斥。然而，第三组（预先照射的异基因大鼠）植入的肿瘤块长得足够大，尽管肿瘤大小的变化小于第一组。还进行了组织学检查。在第一组和第三组中，肾被膜下神经胶质瘤细胞增殖显著，植入的肿瘤组织中的新生血管首先来自被膜，然后来自肾实质。甚至在植入后15天的肿瘤组织中也可见坏死和出血。（摘要截短于250字）
