RNA聚合酶与乳糖阻遏物和乳糖操纵子控制区的相互作用。
The interaction of RNA polymerase and lac repressor with the lac control region.
作者信息
Schmitz A, Galas D J
出版信息
Nucleic Acids Res. 1979 Jan;6(1):111-37. doi: 10.1093/nar/6.1.111.
We have examined the interactions of lac repressor and RNA polymerase with the DNA of the lac control region, using a method for direct visualization of the regions of DNA protected by proteins from DNAase attack. The repressor protects the operator essentially as reported by Gilbert and Maxam (1) with some small modifications. However, the evidence reported here concerning the binding of RNA polymerase to the DNA of the promoter mutant UV5 indicates that : 1) the RNA polymerase molecule binds asymmetrically to the promoter DNA, 2) RNA polymerase protects DNA sequences to within a few bases of the CAP binding site, suggesting direct interaction between polymerase and the CAP protein at this site, 3) RNA polymerase still binds to the promoter when repressor is bound to the operator, but fails to form the same extensive complex.
我们使用一种直接观察受蛋白质保护免受DNA酶攻击的DNA区域的方法，研究了乳糖阻遏物和RNA聚合酶与乳糖操纵子控制区DNA的相互作用。阻遏物对操纵基因的保护作用与吉尔伯特和马克萨姆（1）报道的基本一致，只是有一些小的差异。然而，此处报道的关于RNA聚合酶与启动子突变体UV5的DNA结合的证据表明：1）RNA聚合酶分子与启动子DNA不对称结合；2）RNA聚合酶保护DNA序列直至离CAP结合位点几个碱基处，这表明聚合酶与该位点的CAP蛋白之间存在直接相互作用；3）当阻遏物与操纵基因结合时，RNA聚合酶仍能与启动子结合，但无法形成同样广泛的复合物。
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