Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Orthopedics, The Second Hospital of Shanxi Medical University, Taiyuan, China.
Biotechnol Genet Eng Rev. 2024 Dec;40(4):3047-3063. doi: 10.1080/02648725.2023.2203008. Epub 2023 Apr 20.
Successful bone fragment fixation is a crucial factor in bone fracture healing, the fixation of crushed bone fragments could hinder bone fracture healing. Thus, ideal bone glues to effectively adhere and splice comminuted bone fragments are needed in clinical. Herein, an osteoinductive and biodegradable double cross-linked bone glue (GelMA-oDex-AMBGN) was constructed through Schiff's base reaction between commercial GelMA (with different substitution degrees of amino groups) and Odex mixed with amine-modified mesoporous bioactive glass nanoparticles (AMBGN), followed by crosslink with blue light irradiation. The GelMA-oDex-AMBGN bone glue successfully adhered and spliced the comminuted bone fragments of isolated rat skulls. GelMA-oDex-AMBGN promoted the proliferation of 3T3 cells and enhanced the expression of osteogenic proteins Runx2 and OCN in vitro. In rat cranial critical-sized defect models, GelMA-oDex-AMBGNs with different substitution degrees significantly increased the new bone contents at the fracture defect sites and promoted bone tissue regeneration in vivo. In conclusion, the double cross-linked bone glue (GelMA-oDex-AMBGN) was successfully constructed and can induce bone regeneration. Additionally, there was no significant difference in osteogenic activity among GelMA-oDex-AMBGNs with different substitution degrees and the equal content of AMBGN.
成功的骨碎片固定是骨折愈合的关键因素，粉碎性骨碎片的固定可能会阻碍骨折愈合。因此，临床上需要理想的骨胶来有效黏合和拼接粉碎性骨碎片。在此，通过商业 GelMA（不同取代度的氨基）与混合有胺修饰的介孔生物活性玻璃纳米颗粒（AMBGN）的 Odex 之间的席夫碱反应，构建了一种具有成骨诱导性和可生物降解性的双交联骨胶（GelMA-oDex-AMBGN），然后通过蓝光照射进行交联。GelMA-oDex-AMBGN 成功地黏合和拼接了分离的大鼠颅骨的粉碎性骨碎片。GelMA-oDex-AMBGN 促进了 3T3 细胞的增殖，并增强了体外成骨蛋白 Runx2 和 OCN 的表达。在大鼠颅临界尺寸缺损模型中，不同取代度的 GelMA-oDex-AMBGN 显著增加了骨折缺损部位的新骨含量，并促进了体内骨组织再生。总之，成功构建了双交联骨胶（GelMA-oDex-AMBGN），可诱导骨再生。此外，不同取代度的 GelMA-oDex-AMBGN 之间和成骨活性没有显著差异，且 AMBGN 的含量相等。
