正畸牙齿移动过程中与牙槽骨重塑相关的牙周微小RNA的影响：一项随机临床试验。

Impact of periodontal microRNAs associated with alveolar bone remodeling during orthodontic tooth movement: a randomized clinical trial.

作者信息

Polizzi Alessandro, Alibrandi Angela, Lo Giudice Antonino, Distefano Alfio, Orlando Laura, Analazi Amer M, Pizzo Giuseppe, Volti Giovanni Li, Isola Gaetano

机构信息

Department of General Surgery and Surgical-Medical Specialties, Unit of Periodontology, School of Dentistry, University of Catania, Via S. Sofia 78, Catania, Catania, 95123, Italy.

Department of Economics, Unit of Statistical and Mathematical Sciences, University of Messina, Messina, Italy.

出版信息

J Transl Med. 2024 Dec 30;22(1):1155. doi: 10.1186/s12967-024-05933-x.

DOI:10.1186/s12967-024-05933-x

PMID:39736760

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11684316/

Abstract

BACKGROUND

Micro-RNAs (miRNAs) have been reported to play an important role during orthodontic tooth movement (OTM) through the regulation of periodontal soft and hard tissue homeostasis and functions. The aim of the present study was to assess the effects of miRNAs on OTM and to evaluate possible predictors that influenced the overall OTM amount at a 3-month follow-up.

METHODS

Through a split-mouth design, 21 healthy patients (mean age 13.2 ± 1.8 years) were enrolled in the present study. Clinical parameters and gingival crevicular fluid (GCF) sampling were performed on both compression and tension sides of a random canine to be distalized (test groups) at baseline and at 1 h, 1 day, 1 month and at 3-month after OTM, while the contralateral canine served as a control group. miRNAs - 7a-3p, -7a-2-3p, -7a-5p, -21-3p, -21-5p, -100-3p, -100-5p, -125b-2-3p, -125b-5p, -200b-3p, and - 200b-5p expression was analyzed using a real-time quantitative polymerase chain reaction (RT-PCR). Data were analyzed to assess miRNAs change following OTM. Spearman test, two-way ANOVA and a multivariate regression model were established to evaluate the correlation among miRNAs and clinical parameters and to explore possible predictors of OTM amount at 3-month follow-up.

RESULTS

At 3-month follow-up, there was an increase of miRNA-7a-2-3p, -21-5p, -100-5p, a decrease of miRNA-125b-5p, 200b-3p and - 200b-5p in the compression side and an increase of miRNA-7a-3p, 100-5p in the tension side (p < 0.05). The two-way ANOVA revealed that OTM determined, on the compression side, a significant upregulation on miRNA-7a-3p (p = 0.017), -7a-2-3p (p = 0.023), -21-5p (p = 0.007), -100-5p (p = 0.025) and a significant downregulation of miRNA-125b-2-3p (p = 0.019) and - 200b-5p (p = 0.017). The multivariate model highlighted that high baseline miRNA-7a2-3p (p = 0.025), -21-5p (p = 0.014), -200b-3p (p = 0.041), young age (p = 0.042), lower bleeding on probing (BOP) (p = 0.021) and miRNA-125b-2-3p (p = 0.021) levels were significant predictors of OTM at 3-month follow-up.

CONCLUSIONS

In the present study, OTM significantly impacted the expression of the miRNAs analyzed, in both the tension and compression side of traction tooth at 3-month follow-up. High baseline miRNA-7a2-3p, -21-5p, -200b-3p, and lower miRNA-125b-2-3p, together with younger age and lower BOP, were significant predictors of OTM amount at 3-month follow-up.

TRIAL REGISTRATION

ClinicalTrials.gov NCT06023433 (retrospectively registered).

摘要

背景

据报道，微小RNA（miRNA）通过调节牙周软硬组织的稳态和功能，在正畸牙齿移动（OTM）过程中发挥重要作用。本研究的目的是评估miRNA对OTM的影响，并评估在3个月随访时影响总体OTM量的可能预测因素。

方法

通过双盲设计，本研究纳入了21名健康患者（平均年龄13.2±1.8岁）。在基线以及OTM后1小时、1天、1个月和3个月时，对一颗随机选择的待远中移动的尖牙的压迫侧和张力侧进行临床参数测量和龈沟液（GCF）采样，而对侧尖牙作为对照组。使用实时定量聚合酶链反应（RT-PCR）分析miRNA - 7a - 3p、-7a - 2 - 3p、-7a - 5p、-21 - 3p、-21 - 5p、-100 - 3p、-100 - 5p、-125b - 2 - 3p、-125b - 5p、-200b - 3p和-200b - 5p的表达。分析数据以评估OTM后miRNA的变化。建立Spearman检验、双向方差分析和多元回归模型，以评估miRNA与临床参数之间的相关性，并探索3个月随访时OTM量的可能预测因素。

结果

在3个月随访时，压迫侧miRNA - 7a - 2 - 3p、-21 - 5p、-100 - 5p增加，miRNA - 125b - 5p、200b - 3p和-200b - 5p减少，张力侧miRNA - 7a - 3p、100 - 5p增加（p < 0.05）。双向方差分析显示，OTM在压迫侧导致miRNA - 7a - 3p（p = 0.017）、-7a - 2 - 3p（p = 0.023）、-21 - 5p（p = 0.007）、-100 - 5p（p = 0.025）显著上调，miRNA - 125b - 2 - 3p（p = 0.019）和-200b - 5p（p = 0.017）显著下调。多元模型强调，高基线miRNA - 7a2 - 3p（p = 0.025）、-21 - 5p（p = 0.014）、-200b - 3p（p = 0.041）、年轻（p = 0.042）、较低的探诊出血（BOP）（p = 0.021）和miRNA - 125b - 2 - 3p（p = 0.021）水平是3个月随访时OTM的显著预测因素。