Department of Biology, Aquatic Ecology, Lund University, Lund, Sweden.
Department of Earth Sciences, University of Oxford, Oxford, United Kingdom.
Mol Biol Evol. 2023 May 2;40(5). doi: 10.1093/molbev/msad095.
Sequencing of reduced representation libraries enables genotyping of many individuals for population genomic studies. However, high amounts of DNA are required, and the method cannot be applied directly on single cells, preventing its use on most microbes. We developed and implemented the analysis of single amplified genomes followed by restriction-site-associated DNA sequencing to bypass labor-intensive culturing and to avoid culturing bias in population genomic studies of unicellular eukaryotes. This method thus opens the way for addressing important questions about the genetic diversity, gene flow, adaptation, dispersal, and biogeography of hitherto unexplored species.
简化代表性文库的测序可对许多个体进行基因分型，从而进行群体基因组研究。然而，该方法需要大量的 DNA，并且不能直接应用于单细胞，从而限制了其在大多数微生物上的应用。我们开发并实施了单扩增基因组的分析，随后是限制位点相关的 DNA 测序，以避免繁琐的培养过程，并避免在单细胞真核生物的群体基因组研究中出现培养偏差。因此，该方法为解决有关遗传多样性、基因流动、适应、扩散和生物地理学的重要问题提供了途径，这些问题涉及迄今尚未研究的物种。
