Overstreet D H, Janowsky D S, Gillin J C, Shiromani P J, Sutin E L
Biol Psychiatry. 1986 Jun;21(7):657-64. doi: 10.1016/0006-3223(86)90127-7.
Immobility during forced swimming or after mild footshock (1 mA for 2 sec) was observed in five groups of rats. The Flinders Sensitive Line (FSL) of rats, known to be more sensitive to cholinergic agonists, exhibited the greatest degree of immobility in the forced swim test. Rats chronically treated with, and subsequently withdrawn from, either scopolamine (2 mg/kg, once daily) or amitriptyline (10 mg/kg, once daily) were also significantly more immobile than either a control group treated chronically with isotonic saline or the Flinders Resistant Line (FRL) of rats in the forced swim test. Similar trends were observed for locomotor depression in the open field following exposure to footshock. Receptor binding studies indicated significantly greater concentrations of muscarinic acetylcholine receptors in the hippocampus of the scopolamine, and amitriptyline, withdrawn rats. These findings indicate that rats with increased cholinergic sensitivity are more sensitive to the immobility-inducing effects of mild stressors. Thus, they may prove to be useful models for studying the relationship between affective disorders and the cholinergic system.
在五组大鼠中观察了强迫游泳期间或轻度足部电击（1毫安，持续2秒）后的不动行为。弗林德斯敏感品系（FSL）大鼠已知对胆碱能激动剂更敏感，在强迫游泳试验中表现出最大程度的不动。长期接受东莨菪碱（2毫克/千克，每日一次）或阿米替林（10毫克/千克，每日一次）治疗并随后停药的大鼠，在强迫游泳试验中也比长期接受等渗盐水治疗的对照组或弗林德斯抗性品系（FRL）大鼠明显更不动。暴露于足部电击后，在旷场试验中对运动抑制也观察到类似趋势。受体结合研究表明，东莨菪碱和阿米替林停药大鼠海马中M型胆碱能乙酰胆碱受体浓度显著更高。这些发现表明，胆碱能敏感性增加的大鼠对轻度应激源的致不动效应更敏感。因此，它们可能被证明是研究情感障碍与胆碱能系统之间关系的有用模型。
