Faculty of Pharmacy, DIT University, Dehradun, India.
Non-Wood Forestry Products Division, Forest Research Institute, Dehradun, India.
Women Health. 2023 May-Jun;63(5):359-369. doi: 10.1080/03630242.2023.2197083. Epub 2023 Apr 20.
Ayurvedic system of medicine uses giloy or guduchi, also known as (TC), to treat diabetes and related diseases like hyperglycemia and hyperlipididemia. However, its usage in gestational diabetes mellitus (GDM) is not well studied. The primary objective of the study was to examine the effects of water extract of TC called satva, essential oil, and hydroalcoholic (HA) extract on GDM and its complications and to explore their mechanism of action using mice model. We used streptozotocin-induced diabetes in pregnant mice as murine model and tested TC preparations for anti-GDM activities. Blood glucose, insulin, litter size, and placental weight were assessed. ELISA method was used to measure plasma insulin level to compute homeostatic model assessment of insulin resistance (HOMA-IR), quantitative insulin sensitivity check index (QUICKI), and homeostatic model assessment for assessing beta cell function (HOMA-Beta) levels to estimate insulin resistance, insulin sensitivity, and beta cell function respectively. TC-treated groups had significantly higher serum insulin levels, QUICKI, average litter size, and lower placental weight ( < .001). TC oil and HA extract increased pancreatic beta cell activity according to the level of HOMA-Beta. TC lowered placenta weight and increased litter size significantly compared to control group. Our findings suggest that TC preparations preserve pancreatic beta cells, increase insulin production, decrease insulin resistance, and improve beta cell function, hence preventing GDM. TC preparations also reduced placental weight and increased litter size in mice. Based on these results, we recommend the clinical trial and testing of TC preparations for management of GDM and associated complications. Refer graphical abstract (Figure S1).
阿育吠陀医学系统使用诃子或古德奇，也称为（TC），来治疗糖尿病和相关疾病，如高血糖和高血脂。然而，它在妊娠糖尿病（GDM）中的应用尚未得到充分研究。本研究的主要目的是研究 TC 的水提取物称为 satva、精油和水醇（HA）提取物对 GDM 及其并发症的影响，并使用小鼠模型探索其作用机制。我们使用链脲佐菌素诱导的糖尿病妊娠小鼠作为小鼠模型，测试 TC 制剂的抗 GDM 活性。评估血糖、胰岛素、产仔数和胎盘重量。使用 ELISA 方法测量血浆胰岛素水平，以计算胰岛素抵抗的稳态模型评估（HOMA-IR）、胰岛素敏感性检查指数（QUICKI）和评估β细胞功能的稳态模型评估（HOMA-Beta）水平，以分别估计胰岛素抵抗、胰岛素敏感性和β细胞功能。TC 治疗组的血清胰岛素水平、QUICKI、平均产仔数和胎盘重量均显著升高（<0.001）。TC 油和 HA 提取物根据 HOMA-Beta 水平增加了胰腺β细胞的活性。与对照组相比，TC 显著降低了胎盘重量并增加了产仔数。我们的研究结果表明，TC 制剂可保护胰腺β细胞，增加胰岛素产生，降低胰岛素抵抗，改善β细胞功能，从而预防 GDM。TC 制剂还降低了胎盘重量并增加了小鼠的产仔数。基于这些结果，我们建议进行 TC 制剂治疗 GDM 和相关并发症的临床试验和测试。请参考图形摘要（图 S1）。
