CpG甲基化对2, *和1 G-四链体结构热稳定性的影响。
Effects of CpG methylation on the thermal stability of 2, *, and 1 G-quadruplex structures.
作者信息
Laddachote Saowalak, Ishii Rika, Yoshida Wataru
机构信息
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo, 192-0982, Japan.
Graduate School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo, 192-0982, Japan.
出版信息
BBA Adv. 2021 Mar 20;1:100007. doi: 10.1016/j.bbadva.2021.100007. eCollection 2021.
In genomic DNA, G-quadruplex (G4)-forming DNA can form either a duplex or G4 structure, suggesting that understanding the factors regulating G4 formation is important for revealing the cellular functions controlled by G4 formation. Cytosine DNA methylation in the CpG islands is known to play an important role in transcriptional regulation. Additionally, CpG methylation increases the thermal stability of G4 structures such as and G4. In this study, we evaluated the effects of CpG methylation in three G4 structures (2, *, and 1) produced by the promoter. Each was analyzed using circular dichroism (CD) melting analysis. The results demonstrate that CpG methylation does not alter the thermal stability of 2 G4 structure when formed in the presence of K; a single-CpG methylation at C1 or C11 decreases the thermal stability of any 2 G4 structure formed in the presence of Na and Mg while methylation at C5 increases the thermal stability; CpG methylation does not alter the thermal stability of 1 or * G4 structures formed in the presence of K; and the 1 and * G4-forming oligonucleotides do not form G4 structures in the presence of Na and Mg. These results provide important clues for understanding the regulatory mechanisms underlying the formation of CpG methylation-induced G4 structures.
在基因组DNA中，能够形成G-四链体（G4）的DNA既可以形成双链结构，也可以形成G4结构，这表明了解调控G4形成的因素对于揭示由G4形成所控制的细胞功能至关重要。已知CpG岛中的胞嘧啶DNA甲基化在转录调控中发挥重要作用。此外，CpG甲基化可提高诸如和G4等G4结构的热稳定性。在本研究中，我们评估了由启动子产生的三种G4结构（2、和1）中CpG甲基化的影响。每种结构都使用圆二色性（CD）熔解分析进行了分析。结果表明，当在K存在的情况下形成时，CpG甲基化不会改变2 G4结构的热稳定性；在C1或C11处的单个CpG甲基化会降低在Na和Mg存在的情况下形成的任何2 G4结构的热稳定性，而在C5处的甲基化则会提高热稳定性；CpG甲基化不会改变在K存在的情况下形成的1或 G4结构的热稳定性；并且在Na和Mg存在的情况下，形成1和* G4的寡核苷酸不会形成G4结构。这些结果为理解CpG甲基化诱导的G4结构形成背后的调控机制提供了重要线索。
Zotero 插件