抗疟原虫、抗氧化和细胞毒性活性的一种喀麦隆药用植物用于治疗疟疾。
Antiplasmodial, Antioxidant, and Cytotoxic Activity of a Cameroonian Medicinal Plant Used for the Treatment of Malaria.
机构信息
Department of Animal Organisms, Faculty of Science, University of Douala, P.O. Box 24157, Douala, Cameroon.
Department of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39 Bambili, Cameroon.
出版信息
Biomed Res Int. 2023 Apr 11;2023:1219432. doi: 10.1155/2023/1219432. eCollection 2023.
INTRODUCTION
Resistance to common antimalarial drugs and persistence of the endemicity of malaria constitute a major public health problem in Cameroon. The aim of this study was to evaluate the antiplasmodial, antioxidant, and cytotoxic activities of aqueous and ethanol extracts of used by Cameroonian traditional healers for the treatment of malaria.
METHODS
Aqueous and ethanolic stem bark extracts were prepared according to standard procedures. The SYBR Green method was used for antiplasmodial activity on strains of sensitive to chloroquine (3D7) and resistant (Dd2). antioxidant activities of were determined using the scavenging activity of 2,2'-diphenyl-1-picrylhydrazyl, nitric oxide, ferric reducing power, and hydrogen peroxide as well as their cytotoxicity on RAW 264.7 macrophage cells and red blood cells (RBC).
RESULTS
The aqueous and ethanol extracts of showed antiplasmodial activity on the 3D7 strain with IC of 31.65 ± 0.79 g/ml and 19.41 ± 2.93 g/ml, respectively, as well as 37.64 ± 0.77 g/ml and 36.22 ± 1.04 g/ml for the Dd2 strain, respectively. The aqueous and ethanol extracts showed free radical scavenging properties. The IC aqueous and ethanol extract was approximately 0.0001737 g/ml, 42.92 g/ml, 1197 g/ml, 63.78 g/ml and 4.617 g/ml, 429.9 g/ml, 511 g/ml, and 69.32 g/ml for DPPH, NO, H2O2, and FRAP, respectively, which were compared to ascorbic acid (8.610 - 005 g/ml, 2901 g/ml, 3237 g/ml, and 18.57 g/ml). The aqueous and ethanol extracts of were found to be nontoxic with CC values of 950 ± 6.6 g/ml and 308.3 ± 45.4 g/ml, respectively. Haemolysis test showed that the two extracts were not toxic.
CONCLUSION
These results suggest that can serve as an antimalarial agent. However, further studies are needed to validate the use of as an antimalarial.
简介
抗疟药物的耐药性和疟疾的地方性持续存在是喀麦隆的一个主要公共卫生问题。本研究旨在评估喀麦隆传统医学中用于治疗疟疾的水提物和醇提物的抗疟原虫、抗氧化和细胞毒性活性。
方法
按照标准程序制备水提物和醇提物。采用 SYBR Green 法对氯喹敏感（3D7）和耐药（Dd2）株进行抗疟原虫活性测定。采用 2,2'-二苯基-1-苦基肼、一氧化氮、铁还原能力和过氧化氢的清除活性以及对 RAW 264.7 巨噬细胞和红细胞（RBC）的细胞毒性来测定 的抗氧化活性。
结果
水提物和醇提物对 3D7 株的抗疟原虫活性分别为 IC 为 31.65 ± 0.79 g/ml 和 19.41 ± 2.93 g/ml，对 Dd2 株的抗疟原虫活性分别为 37.64 ± 0.77 g/ml 和 36.22 ± 1.04 g/ml。水提物和醇提物均具有自由基清除能力。水提物和醇提物的 IC 分别约为 0.0001737 g/ml、42.92 g/ml、1197 g/ml、63.78 g/ml 和 4.617 g/ml、429.9 g/ml、511 g/ml 和 69.32 g/ml，与抗坏血酸（8.610 - 005 g/ml、2901 g/ml、3237 g/ml 和 18.57 g/ml）相比。水提物和醇提物对 RBC 的 CC 值分别为 950 ± 6.6 g/ml 和 308.3 ± 45.4 g/ml，表明其无毒性。溶血试验表明，两种提取物均无毒性。
结论
这些结果表明， 可作为抗疟药物。然而，需要进一步的研究来验证 作为抗疟药物的用途。
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