Queensland Brain Institute, University of Queensland, Saint Lucia, Queensland, Australia.
Queensland Centre for Mental Health Research, Wacol, Queensland, Australia.
J Neurochem. 2023 Sep;166(5):779-789. doi: 10.1111/jnc.15829. Epub 2023 May 14.
Vitamin D has been identified as a key factor in dopaminergic neurogenesis and differentiation. Consequently, developmental vitamin D (DVD) deficiency has been linked to disorders of abnormal dopamine signalling with a neurodevelopmental basis such as schizophrenia. Here we provide further evidence of vitamin D's role as a mediator of dopaminergic development by showing that it increases neurite outgrowth, neurite branching, presynaptic protein re-distribution, dopamine production and functional release in various in vitro models of developing dopaminergic cells including SH-SY5Y cells, primary mesencephalic cultures and mesencephalic/striatal explant co-cultures. This study continues to establish vitamin D as an important differentiation agent for developing dopamine neurons, and now for the first time shows chronic exposure to the active vitamin D hormone increases the capacity of developing neurons to release dopamine. This study also has implications for understanding mechanisms behind the link between DVD deficiency and schizophrenia.
维生素 D 已被确定为多巴胺能神经发生和分化的关键因素。因此，发育性维生素 D（DVD）缺乏与具有神经发育基础的异常多巴胺信号传导障碍有关，例如精神分裂症。在这里，我们通过展示维生素 D 增加体外培养的多巴胺能细胞（包括 SH-SY5Y 细胞、原代中脑细胞培养物和中脑/纹状体外植体共培养物）中的神经突生长、神经突分支、突触前蛋白再分布、多巴胺产生和功能释放，提供了维生素 D 作为多巴胺能发育介质的进一步证据。这项研究继续将维生素 D 确立为多巴胺神经元发育的重要分化剂，现在首次表明，慢性暴露于活性维生素 D 激素可增加发育中神经元释放多巴胺的能力。这项研究对于理解 DVD 缺乏与精神分裂症之间联系的背后机制也具有重要意义。
