Liu Xiang, Lei Haibo, Hu Yixiang, Fan Xinyao, Zhang Yazhen, Xie Liyun, Huang Jianji, Cai Qinuo
Department of Clinical Pharmacy, Xiangtan Central Hospital, Xiangtan 411100, PR China.
Department of Clinical Pharmacy, Xiangtan Central Hospital, Xiangtan 411100, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Sep 5;297:122739. doi: 10.1016/j.saa.2023.122739. Epub 2023 Apr 14.
Hydrogen sulfide (HS) is an endogenously generated gaseous signaling molecule and is known to be involved in the occurrence and development of inflammation. To better understand its physiological and pathological process of inflammation, reliable tools for HS detection in living inflammatory models are desired. Although a number of fluorescent sensors have been reported for HS detection and imaging, water-soluble and biocompatibility nanosensors are more useful for imaging in vivo. Herein, we developed a novel biological imaging nanosensor, XNP1, for inflammation-targeted imaging of HS. XNP1 was obtained by self-assembly of amphiphilic XNP1, which was constructed by the condensation reaction of the hydrophobic, HS response and deep red-emitting fluorophore with hydrophilic biopolymer glycol chitosan (GC). Without HS, XNP1 showed very low background fluorescence, while a significant enhancement in the fluorescence intensity of XNP1 was observed in the presence of HS, resulting in a high sensitivity toward HS in aqueous solution with a practical detection limit as low as 32.3 nM, which could be meet the detection of HS in vivo. XNP1 also has a good linear response concentration range (0-1 μM) toward HS with high selectivity over other competing species. These characteristics facilitate direct HS detection of the complex living inflammatory cells and drug-induced inflammatory mice, demonstrating its practical application in biosystems.
硫化氢（HS）是一种内源性产生的气体信号分子，已知其参与炎症的发生和发展。为了更好地理解其炎症的生理和病理过程，需要在活体炎症模型中检测HS的可靠工具。尽管已经报道了许多用于HS检测和成像的荧光传感器，但水溶性和生物相容性纳米传感器在体内成像中更有用。在此，我们开发了一种新型生物成像纳米传感器XNP1，用于HS的炎症靶向成像。XNP1是通过两亲性XNP1的自组装获得的，两亲性XNP1是由疏水性、HS响应性和深红色发射荧光团与亲水性生物聚合物壳聚糖二醇（GC）缩合反应构建而成。在没有HS的情况下，XNP1显示出非常低的背景荧光，而在HS存在下观察到XNP1的荧光强度显著增强，导致其在水溶液中对HS具有高灵敏度，实际检测限低至32.3 nM，这可以满足体内HS的检测。XNP1对HS也具有良好的线性响应浓度范围（0-1 μM），对其他竞争物种具有高选择性。这些特性有助于直接检测复杂的活炎症细胞和药物诱导的炎症小鼠中的HS，证明了其在生物系统中的实际应用。
