饲粮添加二甲基衣康酸酯可缓解慢性热应激对肉鸡生长性能和脂代谢的损害。
Dietary supplementation of dimethyl itaconate protects against chronic heat stress-induced growth performance impairment and lipid metabolism disorder in broiler chickens.
机构信息
Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
出版信息
J Anim Sci. 2023 Jan 3;101. doi: 10.1093/jas/skad120.
This study aimed to investigate the protective effects of dietary supplementation of dimethyl itaconate (DI) on chronic heat stress (HS)-induced impairment of the growth performance and lipid metabolism in broiler chickens. 21 days old male Ross 308 broiler chickens (a total of 120, about 700 g body weight) were randomly divided into five treatment groups, including control group, HS group, HS + 50 mg/kg DI group, HS + 150 mg/kg DI group, and HS + 200 mg/kg DI group, and each group contains eight cages of twenty-four broilers. The broiler chickens in the control group were raised in the room (21 ± 1 °C) and fed with a finisher diet for 21 days. The broiler chickens of the HS group and the HS + DI groups were raised in the room (32 ± 1 °C for 8 h/day) and fed with a finisher diet containing DI at 0, 50, 150, and 200 mg/kg diet for 21 days. The results showed that HS-induced decreases in the final body weight (P < 0.01), average daily gain (P < 0.01), and average daily feed intake (P < 0.01) were alleviated by dietary supplementation of DI (P < 0.05). In addition, dietary supplementation of DI attenuated the increases in the liver index (P < 0.01) and abdominal fat rate (P < 0.01) caused by HS in broilers (P < 0.05). Treatment with DI ameliorated HS-induced lipid accumulation in the liver and serum of broiler chickens (P < 0.05). The upregulation of mRNA levels of fat synthesis factors (P < 0.01) and downregulation of mRNA levels of lipolysis-related factors (P < 0.01) caused by HS were markedly blunted after treatment with DI in the liver of broilers (P < 0.05). Broilers exposed to HS exhibited lower phosphorylated protein levels of AMP-activated protein kinase α and acetyl-CoA carboxylase α compared to the control group (P < 0.01), which were improved by treatment with DI (P < 0.01). Collectively, these results demonstrated that dietary supplementation of DI protects against chronic HS-induced growth performance impairment and lipid metabolism disorder in broiler chickens. These results not only provide a theoretical basis for DI to alleviate metabolic disorders but also provide a reference value for DI as a feed additive to improve heat stress in poultry caused by high temperature.
本研究旨在探讨二甲基衣康酸（DI）膳食补充对慢性热应激（HS）诱导的肉鸡生长性能和脂质代谢损伤的保护作用。将 21 日龄雄性罗斯 308 肉鸡（共 120 只，体重约 700g）随机分为 5 个处理组，包括对照组、HS 组、HS+50mg/kg DI 组、HS+150mg/kg DI 组和 HS+200mg/kg DI 组，每组 8 个笼，每个笼 24 只肉鸡。对照组肉鸡在室内（21±1°C）饲养，21 天内饲喂育肥鸡饲料。HS 组和 HS+DI 组肉鸡在室内（每天 8 小时 32±1°C）饲养，饲料中添加 0、50、150 和 200mg/kg 日粮 DI，21 天。结果表明，HS 诱导的终体重降低（P<0.01）、平均日增重（P<0.01）和平均日采食量降低（P<0.01）被 DI 膳食补充缓解（P<0.05）。此外，DI 处理减轻了 HS 引起的肉鸡肝脏指数（P<0.01）和腹脂率（P<0.01）的增加（P<0.05）。DI 处理改善了 HS 引起的肉鸡肝脏和血清脂质堆积（P<0.05）。HS 诱导的肉鸡肝脏脂肪合成因子 mRNA 水平上调（P<0.01）和脂肪分解相关因子 mRNA 水平下调（P<0.01）被 DI 处理显著减弱（P<0.05）。HS 暴露的肉鸡 AMP 激活蛋白激酶α和乙酰辅酶 A 羧化酶α磷酸化蛋白水平低于对照组（P<0.01），经 DI 处理后有所改善（P<0.01）。综上所述，膳食补充 DI 可防止慢性 HS 诱导的肉鸡生长性能下降和脂质代谢紊乱。这些结果不仅为 DI 缓解代谢紊乱提供了理论依据，也为 DI 作为饲料添加剂改善高温引起的家禽热应激提供了参考价值。
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