College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, People's Republic of China.
School of Animal Science and Food Engineering, Institute of Jingling Technology, Nanjing, People's Republic of China.
J Sci Food Agric. 2023 Mar 30;103(5):2367-2377. doi: 10.1002/jsfa.12431. Epub 2023 Jan 18.
This study was conducted to investigate the effect of dietary rutin on the meat quality, antioxidant status and mitochondrial structure and function in the breast muscle of heat-stressed broilers. A total of 192 male broilers were randomly assigned into three groups and treated with normal control (CON), heat stress (34 °C, HS), and HS with 500 mg kg rutin supplementation (HS + Rutin), respectively.
Dietary rutin significantly reversed HS-induced decrease in body weight, average daily feed intake, average daily gain, and feed efficiency. Rutin supplementation attenuated HS-induced impaired meat quality by decreasing the lightness, drip loss at 24 and 48 h, the peak time of free water (T ) and the peak area ratio of free water (P ), and increasing the pH and peak area ratio of immobilized water (P ). Rutin supplementation promoted superoxide dismutase, glutathione peroxidase activities and total antioxidant capacity, and decreased malondialdehyde levels compared with the HS group. Moreover, rutin attenuated HS-induced mitochondrial damage by increasing the mitochondrial DNA copy number and improving mitochondrial morphology. Dietary rutin significantly increased mitochondrial biogenesis-related mRNA (proliferator-activated γ receptor coactivator-1α [PGC-1α], nuclear respiratory factor 1 [NRF1], and mitochondrial transcription factor A [TFAM]) expression via the AMP-activated protein kinase (AMPK) signaling pathway. HS significantly increased mitophagy-related genes and proteins (Parkin, PTEN-induced putative kinase 1 [PINK1], microtubule associated protein light chain 3-II [LC3-II]) expression, and dietary rutin significantly reversed these alterations.
Dietary rutin attenuated the HS-induced decline in meat quality and antioxidant capacity of broilers, which may be related to inhibition of the AMPK/PINK1-Parkin signaling pathway to attenuate mitochondrial damage. © 2023 Society of Chemical Industry.
本研究旨在探讨日粮芦丁对热应激肉鸡胸肌肉质、抗氧化状态和线粒体结构与功能的影响。将 192 只雄性肉鸡随机分为三组，分别给予正常对照（CON）、热应激（34°C，HS）和 HS 加 500mg/kg 芦丁补充（HS+Rutin）处理。
日粮芦丁显著逆转了 HS 引起的体重、平均日采食量、平均日增重和饲料效率下降。芦丁补充通过降低亮度、24 和 48 小时的滴水损失、游离水的峰值时间（T）和游离水的峰值面积比（P），以及提高 pH 和固定水的峰值面积比（P），减轻了 HS 引起的肉质变差。与 HS 组相比，芦丁补充提高了超氧化物歧化酶、谷胱甘肽过氧化物酶活性和总抗氧化能力，降低了丙二醛水平。此外，芦丁通过增加线粒体 DNA 拷贝数和改善线粒体形态，减轻了 HS 引起的线粒体损伤。日粮芦丁通过激活 AMP 激活蛋白激酶（AMPK）信号通路，显著增加了与线粒体生物发生相关的 mRNA（过氧化物酶体增殖物激活受体γ共激活因子 1α[PGC-1α]、核呼吸因子 1[NRF1]和线粒体转录因子 A[TFAM]）表达。HS 显著增加了与线粒体自噬相关的基因和蛋白（Parkin、PTEN 诱导的假定激酶 1[PINK1]、微管相关蛋白轻链 3-II[LC3-II]）的表达，而日粮芦丁显著逆转了这些变化。
日粮芦丁减轻了 HS 引起的肉鸡肉质和抗氧化能力下降，这可能与抑制 AMPK/PINK1-Parkin 信号通路减轻线粒体损伤有关。
