Einhorn L, Mitrani E
Cell Differ. 1986 May;18(3):153-6. doi: 10.1016/0045-6039(86)90080-1.
Early chick embryonic cells, prior to the formation of the primitive streak, form colonies when cultured in soft agarose [Mitrani, E.: Exp. Cell Res. 152, 148-153 (1984)]. The present work is an attempt to determine at which stages of development this ability is expressed and which areas of the chick embryo harbour the colony-forming cells. We found that the capacity to form colonies decreases as development progresses and cells enter alternative differentiation pathways. At pre-primitive streak stages, the capacity is concentrated to the peripheral areas of the embryo and decreases towards the centre. With the onset of hypoblast formation only cells from Area Opaca and, to a lesser degree, the Marginal Zone, can form colonies in agarose. At post-primitive streak stages only extra-embryonic cells can form colonies in agarose. By 48 h of incubation all cells of the chick blastoderm seem to have lost the capacity to form colonies in agarose.
在原条形成之前，早期鸡胚细胞在软琼脂糖中培养时会形成集落[米特拉尼，E.：《实验细胞研究》152，148 - 153（1984）]。本研究旨在确定这种能力在发育的哪些阶段表达，以及鸡胚的哪些区域含有集落形成细胞。我们发现，随着发育的进行和细胞进入其他分化途径，形成集落的能力会下降。在原条形成前阶段，这种能力集中在胚胎的周边区域，并向中心递减。随着下胚层形成的开始，只有来自明区的细胞，以及程度较轻的边缘区细胞，能够在琼脂糖中形成集落。在原条形成后阶段，只有胚外细胞能够在琼脂糖中形成集落。孵化48小时后，鸡胚盘的所有细胞似乎都失去了在琼脂糖中形成集落的能力。
