Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Medicine, School of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, PR China.
State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.
Talanta. 2023 Aug 1;260:124552. doi: 10.1016/j.talanta.2023.124552. Epub 2023 Apr 11.
Previous researches have suggested the potential correlation between the development of breast cancer and the concentration of miRNA-21 in serum. Theoretically the doping of multivalent metal ions in WS could bring higher electron transfer capacity, but this hasn't been proven. To fill this research gap, through one-pot method we prepared seven nanocomposite structures modified with different metal ions (Co, Ni, Mn, Zn, Fe, Cr, La). Characterization revealed that ammonia produced by hydrothermal urea exfoliated the multilayer graphene oxide (MGO) and provided a nitrogen source for doping reduction to form a 3D flower-like structure (NrGOF) with high specific surface area. Meanwhile, the modification of WS by Fe not only enhanced its electrochemical conductivity but also gave the material an additional peroxidase activity centre. In the composite Fe-WS/NrGOF-AgNPs, NrGOF is used as a conductive loading interface for WS, while Fe served as the catalytic and electron transfer centre for secondary amplification of the electrochemical signal. The experimental results showed that the sensing platform has a low limit of detection (LOD) of 1.18 aM for miRNA-21 in the concentration range of 10-10 M and has been successfully applied to the detection of real serum samples.
先前的研究表明，乳腺癌的发展与血清中 microRNA-21 的浓度之间存在潜在的相关性。理论上，多价金属离子掺杂到 WS 中可以带来更高的电子转移能力，但这尚未得到证实。为了填补这一研究空白，我们通过一锅法制备了七种用不同金属离子（Co、Ni、Mn、Zn、Fe、Cr、La）修饰的纳米复合材料结构。表征结果表明，水热尿素产生的氨将多层氧化石墨烯（MGO）剥离，并为掺杂还原提供了氮源，从而形成具有高比表面积的 3D 花状结构（NrGOF）。同时，Fe 对 WS 的修饰不仅增强了其电化学导电性，还为材料提供了额外的过氧化物酶活性中心。在复合 Fe-WS/NrGOF-AgNPs 中，NrGOF 用作 WS 的导电负载界面，而 Fe 则用作电化学信号二次放大的催化和电子转移中心。实验结果表明，该传感平台对浓度范围为 10-10 M 的 microRNA-21 的检测下限（LOD）低至 1.18 aM，并已成功应用于实际血清样品的检测。
