Ren Jifan, Wang Qingji, Zuo Jie, Jiang Shuxia
a College of Plant Protection, Shandong Agricultural University, Shandong Province Key Lab. of Agricultural Microorganisms.
b College of Plant Protection, Shandong Agricultural University, Experimental Center.
Mycoscience. 2021 Mar 20;62(2):95-105. doi: 10.47371/mycosci.2020.11.006. eCollection 2021.
is a popular edible mushroom in the world. High temperature seriously affects its yield and quality. In this study, transcriptome sequencing was performed on the mycelia of heat-resistant strains and heat-sensitive strains heat-treated at 38 °C for 0 h and 24 h. The changes of catalase (CAT) activity, superoxide dismutase (SOD) activity and trehalose content in the mycelia under high temperature stress were also measured and analyzed. We find that the differential genes are mainly enriched in the pathways of glycerophospholipid metabolism, starch and sucrose metabolism, protein processing in the endoplasmic reticulum, etc. The expression levels of genes encoding trehalose-6-phosphate phosphatase (TPP), CAT, SOD, etc. are quite different. And these genes' variation range in the thermotolerant strain are higher than that in heat-sensitive strain. The CAT activity and trehalose content of the two strains increase first and then decrease, and the SOD activity increase slowly. The CAT, SOD activity and trehalose content of the thermotolerant strain are higher than those of the heat-sensitive strain. This study will provide a basis for further research on important signal pathways and gene function identification of related to high temperature stress.
是世界上一种受欢迎的可食用蘑菇。高温严重影响其产量和品质。在本研究中，对耐热菌株和热敏菌株的菌丝体进行转录组测序，这些菌丝体在38℃下分别热处理0小时和24小时。还测定并分析了高温胁迫下菌丝体中过氧化氢酶（CAT）活性、超氧化物歧化酶（SOD）活性和海藻糖含量的变化。我们发现差异基因主要富集在甘油磷脂代谢、淀粉和蔗糖代谢、内质网中的蛋白质加工等途径中。编码海藻糖-6-磷酸磷酸酶（TPP）、CAT、SOD等的基因表达水平差异较大。并且这些基因在耐热菌株中的变化范围高于热敏菌株。两菌株的CAT活性和海藻糖含量先升高后降低，SOD活性缓慢升高。耐热菌株的CAT、SOD活性和海藻糖含量高于热敏菌株。本研究将为进一步研究与高温胁迫相关的重要信号通路和基因功能鉴定提供依据。
