Ganaparthi Venkata Rao, Adhikari Sagar, Marais Francois, Neupane Bipin, Bisek Bradley
Plant Sciences Department, North Dakota State University, Fargo, ND, 58108, USA.
Coastal Research and Education Center, Clemson University, Charleston, SC, USA.
Heliyon. 2023 Apr 5;9(4):e15103. doi: 10.1016/j.heliyon.2023.e15103. eCollection 2023 Apr.
Fusarium head blight (FHB) caused by results in substantial grain yield and quality losses in common wheat ( L.). Genetic resistance is partial but crucial for effective, integrated management of the disease. Host resistance is conditioned by numerous small effect quantitative trait loci (QTL) that are strongly affected by the environment and genetic background. and (PI 277012 is the source for both genes) are two recently discovered FHB resistance QTL that also occur in spring wheat GP80 (PI 277012 derivative). To transfer the PI 277012 resistance from GP80 to hard winter wheat (HWW), GP80 was first crossed with Novus-4. The F hybrid was crossed with SY Monument, following which marker-selected progeny were crossed with, and backcrossed to, ND Noreen. To potential carriers of FHB resistance QTL among the 22 F of the ND Noreen cross, simple sequence repeat (SSR) markers, Illumina 90 K single nucleotide polymorphism (SNP) haplotypes and greenhouse FHB Type II resistance tests were done. Likely homozygotes for and , were selected and backcrossed to ND Noreen. In the BF, 131 plants were evaluated for SNP haplotypes, SSR markers and FHB resistance. Nine BF lines were derived, and their resistance confirmed in a third greenhouse FHB trial. The results suggested that eight lines had higher resistance and were comparable to GP80 with the markers occurring in all eight and the markers occurring in four lines. The eight selections constitute a valuable HWW resistance breeding resource.
由镰刀菌引起的小麦赤霉病（FHB）会导致普通小麦（Triticum aestivum L.）的谷物产量和品质大幅下降。遗传抗性虽不彻底，但对该病的有效综合管理至关重要。寄主抗性由众多效应较小的数量性状位点（QTL）决定，这些位点受环境和遗传背景的影响很大。Fhb1和Fhb5（PI 277012是这两个基因的来源）是最近发现的两个抗小麦赤霉病QTL，它们也存在于春小麦GP80（PI 277012的衍生品种）中。为了将GP80中PI 277012的抗性转移到硬粒冬小麦（HWW）中，首先将GP80与Novus-4杂交。F1杂种与SY Monument杂交，之后将标记选择的后代与ND Noreen杂交并回交。对ND Noreen杂交的22个F2代中潜在的小麦赤霉病抗性QTL携带者，进行了简单序列重复（SSR）标记、Illumina 90K单核苷酸多态性（SNP）单倍型分析和温室小麦赤霉病II型抗性测试。选择了可能的Fhb1和Fhb5纯合子并与ND Noreen回交。在BC1F1代中，对131株植株进行了SNP单倍型、SSR标记和小麦赤霉病抗性评估。获得了9个BC1F1代株系，并在第三次温室小麦赤霉病试验中证实了它们的抗性。结果表明，8个株系具有较高的抗性，与GP80相当，所有8个株系都有Fhb1标记，4个株系有Fhb5标记。这8个选择系构成了宝贵的硬粒冬小麦抗性育种资源。
