Mbindyo Sharon N, Kitaa Jafred M A, Abuom Tequiero O, Aboge Gabriel O, Muasya Daniel W, Muchira Beatrice W, Gitahi Nduhiu, Mulei Charles M
Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, P.O. Box 29053-00625, Kangemi, Kenya.
Department of Public Health, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi, P.O. Box 29053-00625, Kangemi, Kenya.
Vet Med Int. 2023 Apr 8;2023:8813405. doi: 10.1155/2023/8813405. eCollection 2023.
species are widely distributed pathogens; however, data on its epidemiology in puppies remain scanty, especially in Kenya. A cross-sectional study was conducted in the Nairobi Metropolitan Region to determine molecular prevalence and associated risk factors of species infection in puppies. A total of 260 rectal swabs were collected from puppies from breeding kennels, shelters, and the University of Nairobi Veterinary Teaching and Referral Hospital. The samples were subjected to polymerase chain reaction (PCR) assays for identification of species. Data on potential risk factors associated with puppy exposure were collected using a semistructured questionnaire. Multivariable mixed effects logistic regression analyses were performed with kennels as random effects. species were detected in 64 of the 260 sampled puppies yielding an overall prevalence of 24.6%. Multivariable results showed that puppies from shelters, puppies from kennels that are washed daily, puppies with a recent history of vomiting, and those treated with antibiotics in the past month were significantly associated with the presence of species. Being a kenneled puppy and having had concurrent bacterial infections were identified as protective factors. This study provides molecular evidence of puppy exposure to species which could have impact on puppy health and highlights the need to develop awareness and management strategies to potentially reduce the risk of transmitting this pathogen among puppies, to humans, and other animals.
[病原体名称]是广泛分布的病原体；然而，关于其在幼犬中的流行病学数据仍然很少，尤其是在肯尼亚。在内罗毕大都会地区进行了一项横断面研究，以确定幼犬感染[病原体名称]的分子流行率及相关风险因素。总共从繁殖犬舍、收容所和内罗毕大学兽医教学与转诊医院的幼犬中采集了260份直肠拭子。对样本进行聚合酶链反应（PCR）检测以鉴定[病原体名称]。使用半结构化问卷收集与幼犬接触相关的潜在风险因素的数据。以犬舍为随机效应进行多变量混合效应逻辑回归分析。在260只采样幼犬中，有64只检测出[病原体名称]，总体流行率为24.6%。多变量结果显示，来自收容所的幼犬、每天清洗的犬舍中的幼犬、近期有呕吐史的幼犬以及在过去一个月内接受过抗生素治疗的幼犬与[病原体名称]的存在显著相关。作为圈养幼犬和曾有过并发细菌感染被确定为保护因素。本研究提供了幼犬接触[病原体名称]的分子证据，这可能会对幼犬健康产生影响，并强调需要制定提高认识和管理策略，以潜在降低该病原体在幼犬之间、向人类和其他动物传播的风险。
