Kitahara Michiru, Nagamune Kosuke, Kinoshita Akihiko, Yugeta Chigusa, Ohara Naoto, Shimazaki Akane, Yamashita Yumi, Yukawa Tomohisa, Endo Naoki, Ogura-Tsujita Yuki
a Faculty of Agriculture, Saga University.
b Graduate School of Sustainability Science, Tottori University.
Mycoscience. 2022 May 31;63(3):88-95. doi: 10.47371/mycosci.2022.04.001. eCollection 2022.
We performed germination tests on seeds from five orchids (, var. , var. , , and ) using one and two isolates. sp. 1 promoted germination of all five orchids, with root and/or tuber formation observed in , , and . No additional growth was observed in the other two orchids. sp. 2 induced , var. , and germination, whereas sp. 1 induced and germination. Phylogenetic analyses indicated that the two isolates represent distinct lineages within the . sp. 1 and sp. 1 are closely related to and , respectively. Our results imply that and marasmioid fungi play important roles in early development in species, and that fungi in particular may be common mycobionts of species. Root and/or tuber development was observed with four plant-fungus combinations, implying that these associations persist throughout the life cycle, whereas var. may require different associates over time. Our findings will contribute to elucidating the mycorrhizal associations of mycoheterotrophic orchids throughout their life cycle.
我们使用一种和两种分离菌株对五种兰花（、变种、变种、和）的种子进行了发芽试验。菌株1促进了所有五种兰花的发芽，在、和中观察到了根和/或块茎的形成。在另外两种兰花中未观察到额外的生长。菌株2诱导了、变种和的发芽，而菌株1诱导了和的发芽。系统发育分析表明，这两种分离菌株代表了属内不同的谱系。菌株1和菌株1分别与和密切相关。我们的结果表明，和类层腹菌纲真菌在物种的早期发育中起重要作用，特别是真菌可能是物种常见的菌根共生体。在四种植物 - 真菌组合中观察到了根和/或块茎的发育，这意味着这些共生关系在整个生命周期中持续存在，而变种可能随着时间的推移需要不同的共生伙伴。我们的发现将有助于阐明菌根异养兰花在其整个生命周期中的菌根共生关系。
