Higham D P, Sadler P J, Scawen M D
Environ Health Perspect. 1986 Mar;65:5-11. doi: 10.1289/ehp.86655.
Pseudomonas putida adapted to growth in 3 mM cadmium. The resistance mechanism involved complexation of cadmium in polyphosphate granules, changes in the structure of the cell membrane and induction of three cysteine-rich, low molecular weight proteins (3500-7000) containing 4 to 7 g-atoms per mole of cadmium, zinc, and copper. Each protein was produced during a different phase of growth, and the smallest protein (3500) was released into the environment when the cells lysed at the end of the exponential phase. The metal binding sites of the major protein were further characterized using a range of physical methods, including 113Cd NMR. The properties of the bacterial pseudothioneins are compared to those of metallothioneins.
恶臭假单胞菌适应在3 mM镉环境中生长。其抗性机制包括镉在多聚磷酸盐颗粒中的络合、细胞膜结构的变化以及三种富含半胱氨酸的低分子量蛋白质（3500 - 7000）的诱导产生，这些蛋白质每摩尔含有4至7克原子的镉、锌和铜。每种蛋白质在不同的生长阶段产生，最小的蛋白质（3500）在指数期末细胞裂解时释放到环境中。使用包括113Cd NMR在内的一系列物理方法进一步表征了主要蛋白质中的金属结合位点。将细菌假硫蛋白的特性与金属硫蛋白的特性进行了比较。
