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本文引用的文献

1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
THE ZINC-BINDING GROUPS OF CARBOXYPEPTIDASE A.羧肽酶A的锌结合基团
Biochemistry. 1964 May;3:653-62. doi: 10.1021/bi00893a010.
3
Metallothionein: a cadmium and zinc-containign protein from equine renal cortex. II. Physico-chemical properties.金属硫蛋白：一种来自马肾皮质的含镉和锌的蛋白质。II. 物理化学性质。
J Biol Chem. 1961 Sep;236:2435-42.
4
Metallothionein: a cadmium- and zinc-containing protein from equine renal cortex.金属硫蛋白：一种来自马肾皮质的含镉和锌的蛋白质。
J Biol Chem. 1960 Dec;235:3460-5.
5
A study of the cytochrome c haemochromogen.细胞色素c血色原的研究。
Biochem J. 1959 Mar;71(3):559-70. doi: 10.1042/bj0710559.
6
The sulfhydryl groups of crystalline proteins. I. Some albumins, enzymes, and hemoglobins.结晶蛋白质的巯基。I. 一些白蛋白、酶和血红蛋白。
J Biol Chem. 1955 Oct;216(2):663-76.
7
Isolation and some properties of human metallothionein.人金属硫蛋白的分离及某些特性
Biochemistry. 1966 May;5(5):1768-77. doi: 10.1021/bi00869a046.
8
Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.通过圆盘凝胶电泳进行蛋白质的大小和电荷异构体分离及分子量估计。
Arch Biochem Biophys. 1968 Jul;126(1):155-64. doi: 10.1016/0003-9861(68)90569-9.
9
Separation of two forms of rabbit metallothionein by isoelectric focusing.通过等电聚焦分离兔金属硫蛋白的两种形式。
Biochem J. 1972 Feb;126(3):491-8. doi: 10.1042/bj1260491.
10
Equine hepatic and renal metallothioneins. Purification, molecular weight, amino acid composition, and metal content.马肝脏和肾脏中的金属硫蛋白。纯化、分子量、氨基酸组成及金属含量。
J Biol Chem. 1974 Jun 10;249(11):3537-42.

鲽金属硫蛋白的纯化及特性，一种来自鲽（欧洲比目鱼）肝脏的镉结合蛋白。

Purification and properties of plaice metallothionein, a cadmium-binding protein from the liver of the plaice (Pleuronectes platessa).

作者信息

Overnell J, Coombs T L

出版信息

Biochem J. 1979 Nov 1;183(2):277-83. doi: 10.1042/bj1830277.

DOI:10.1042/bj1830277
PMID:534496
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1161556/
Abstract

A low-molecular-weight protein induced in the liver of the plaice (Pleuronectes platessa) by exposure to cadmium was purified and characterized. It is closely similar to mammalian metallothioneins in all of its properties in that it is a single-chain cadmium-binding protein of approx. 7000 mol.wt. with a high cysteine content (31 mol%) and no aromatic amino acid residues. The thiol groups of the cysteine residues complex with the cadmium in a SH/Cd molar ratio of 3:1 and produce a characteristic absorption maximum at 250 nm. Unlike the mammalian metallothioneins, however, metal analyses reveal only traces of zinc and copper in addition to cadmium. The presence of carbohydrate previously assumed from a positive reaction with periodic acid/Schiff reagent has now been disproved, and the positive reaction attributed to interaction with the thiol groups in the protein.

摘要

通过镉暴露在欧鳎（Pleuronectes platessa）肝脏中诱导产生的一种低分子量蛋白质被纯化并进行了特性鉴定。它在所有特性上都与哺乳动物金属硫蛋白非常相似，即它是一种单链镉结合蛋白，分子量约为7000道尔顿，半胱氨酸含量高（31摩尔%）且无芳香族氨基酸残基。半胱氨酸残基的巯基与镉以3:1的SH/Cd摩尔比络合，并在250纳米处产生特征性吸收峰。然而，与哺乳动物金属硫蛋白不同的是，金属分析表明除镉外仅含有痕量的锌和铜。先前通过与高碘酸/席夫试剂的阳性反应推测存在的碳水化合物现在已被证伪，阳性反应归因于与蛋白质中巯基的相互作用。