D'Halluin Alexandre, Polgar Peter, Kipkorir Terry, Patel Zaynah, Cortes Teresa, Arnvig Kristine B
Structural and Molecular Biology, University College London, London WC1E 6BT, UK.
Instituto de Biomedicina de Valencia, CSIC, Valencia 46010, Spain.
iScience. 2023 Mar 22;26(4):106465. doi: 10.1016/j.isci.2023.106465. eCollection 2023 Apr 21.
Little is known about the decisions behind transcription elongation versus termination in the human pathogen . By applying Term-seq to we found that the majority of transcription termination is premature and associated with translated regions, i.e., within previously annotated or newly identified open reading frames. Computational predictions and Term-seq analysis, upon depletion of termination factor Rho, suggests that Rho-dependent transcription termination dominates all transcription termination sites (TTS), including those associated with regulatory 5' leaders. Moreover, our results suggest that tightly coupled translation, in the form of overlapping stop and start codons, may suppress Rho-dependent termination. This study provides detailed insights into novel .-regulatory elements, where Rho-dependent, conditional termination of transcription and translational coupling together play major roles in gene expression control. Our findings contribute to a deeper understanding of the fundamental regulatory mechanisms that enable . adaptation to the host environment offering novel potential points of intervention.
对于人类病原体中转录延伸与终止背后的决定因素，我们知之甚少。通过将Term-seq应用于……，我们发现大多数转录终止是过早的，并且与翻译区域相关，即在先前注释或新鉴定的开放阅读框内。在终止因子Rho缺失时进行的计算预测和Term-seq分析表明，Rho依赖性转录终止主导所有转录终止位点（TTS），包括那些与调控性5'前导序列相关的位点。此外，我们的结果表明，以重叠的终止密码子和起始密码子形式存在的紧密偶联翻译可能会抑制Rho依赖性终止。这项研究提供了对新型……调控元件的详细见解，其中Rho依赖性、条件性转录终止和翻译偶联在基因表达控制中共同发挥主要作用。我们的发现有助于更深入地理解使……能够适应宿主环境的基本调控机制，从而提供新的潜在干预点。
