Xu Wenlin, Qi Haoran, Shen Tengfei, Zhao Meiqi, Song Zihe, Ran Na, Wang Jiali, Xi Mengli, Xu Meng
Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing, China.
iScience. 2023 Mar 24;26(4):106496. doi: 10.1016/j.isci.2023.106496. eCollection 2023 Apr 21.
Poplar coma, commonly referred to as "seed hairs", is a tuft of trichomes attached to the seed coat that helps seed dispersal. However, they can also trigger health impacts for humans, including sneezing, shortness of breath, and skin irritation. Despite efforts to study the regulatory mechanism of herbaceous trichome formation, poplar coma remains poorly understood. In this study, we showed that the epidermal cells of the funiculus and placenta are the origin of poplar coma based on observations of paraffin sections. Small RNA (sRNA) and degradome libraries were also constructed at three stages of poplar coma development, including initiation and elongation stages. Based on 7,904 miRNA-target pairs identified by small RNA and degradome sequencing, we constructed a miRNA-transcript factor and a stage-specific miRNA regulatory network. By combining paraffin section observation and deep sequencing, our research will provide greater insight into the molecular mechanisms of poplar coma development.
杨树柔毛，通常被称为“种毛”，是附着在种皮上的一簇毛状体，有助于种子传播。然而，它们也会对人类健康产生影响，包括打喷嚏、呼吸急促和皮肤过敏。尽管人们努力研究草本植物毛状体形成的调控机制，但杨树柔毛仍知之甚少。在本研究中，基于石蜡切片观察，我们表明珠柄和胎座的表皮细胞是杨树柔毛的起源。还在杨树柔毛发育的三个阶段，包括起始和伸长阶段，构建了小RNA（sRNA）和降解组文库。基于通过小RNA和降解组测序鉴定的7904个miRNA-靶标对，我们构建了一个miRNA-转录因子和一个阶段特异性miRNA调控网络。通过结合石蜡切片观察和深度测序，我们的研究将为杨树柔毛发育的分子机制提供更深入的见解。
