整合降解组和 Srna 测序揭示了杨树韧皮部和木质部发育过程中 miRNA-mRNA 调控网络。

Integrated Degradome and Srna Sequencing Revealed miRNA-mRNA Regulatory Networks between the Phloem and Developing Xylem of Poplar.

机构信息

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Int J Mol Sci. 2022 Apr 20;23(9):4537. doi: 10.3390/ijms23094537.

DOI:10.3390/ijms23094537

PMID:35562928

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9100975/

Abstract

Lignin and cellulose are the most abundant natural organic polymers in nature. MiRNAs are a class of regulatory RNAs discovered in mammals, plants, viruses, and bacteria. Studies have shown that miRNAs play a role in lignin and cellulose biosynthesis by targeting key enzymes. However, the specific miRNAs functioning in the phloem and developing xylem of are still unknown. In this study, a total of 134 miRNAs were identified via high-throughput small RNA sequencing, including 132 known and two novel miRNAs, six of which were only expressed in the phloem. A total of 58 differentially expressed miRNAs (DEmiRNAs) were identified between the developing xylem and the phloem. Among these miRNAs, 21 were significantly upregulated in the developing xylem in contrast to the phloem and 37 were significantly downregulated. A total of 2431 target genes of 134 miRNAs were obtained via high-throughput degradome sequencing. Most target genes of these miRNAs were transcription factors, including , , , , , , , , , and genes. Furthermore, 13 and nine miRNAs were involved in lignin and cellulose biosynthesis, respectively, and we validated the miRNAs via qRT-PCR. Our study explores these miRNAs and their regulatory networks in the phloem and developing xylem of and provides new insight into wood formation.

摘要

木质素和纤维素是自然界中最丰富的天然有机聚合物。miRNAs 是一类在哺乳动物、植物、病毒和细菌中发现的调节 RNA。研究表明，miRNAs 通过靶向关键酶在木质素和纤维素生物合成中发挥作用。然而，在韧皮部和发育中的木质部中起作用的特定 miRNAs 仍然未知。在这项研究中，通过高通量小 RNA 测序共鉴定出 134 个 miRNAs，包括 132 个已知和 2 个新的 miRNAs，其中 6 个仅在韧皮部中表达。在发育中的木质部和韧皮部之间共鉴定出 58 个差异表达的 miRNAs（DEmiRNAs）。在这些 miRNAs 中，有 21 个在发育中的木质部中显著上调，而 37 个显著下调。通过高通量降解组测序共获得 134 个 miRNAs 的 2431 个靶基因。这些 miRNAs 的大多数靶基因是转录因子，包括、、、、、、、和基因。此外，有 13 个和 9 个 miRNAs 分别参与木质素和纤维素的生物合成，我们通过 qRT-PCR 对这些 miRNAs 进行了验证。我们的研究探索了这些 miRNAs 及其在韧皮部和发育中的木质部中的调控网络，为木材形成提供了新的见解。