College of Forestry, Northeast Forestry University, Hexing Road 26, Xiangfang District, Harbin, 150040, Heilongjiang, China.
College of Forestry and Grassland Science, Jilin Agricultural University, Xincheng Street 2888, Changchun, 130118, Jilin, China.
Microb Biotechnol. 2023 Jun;16(6):1360-1372. doi: 10.1111/1751-7915.14262. Epub 2023 Apr 25.
Sanghuangporus baumii is a medicinal fungi with anti-inflammatory, liver protection and antitumour effects. Terpenoids are one of the main medicinal ingredients of S. baumii. However, terpenoid production by wild-type S. baumii cannot meet the market demand, which affects its application in medical care. Therefore, exploring how to increase terpenoid content in S. baumii is a promising path in this research field. Salicylic acid (SA) is a secondary metabolite. In this study, a concentration of 350 μmol/L SA was added into fungal cultivations for 2 and 4 days, and then the transcriptome and metabolome of untreated mycelia and treated with SA were analysed. The expression of some genes in the terpenoids biosynthesis pathway increased in SA-induced cultivations, and the content of isopentenyl pyrophosphate (IPP) and geranylgeranyl-PP (GGPP) increased significantly as well as the contents of triterpenoids, diterpenoids, sesquiterpenoids and carotenoids. The gene FPS was considered to be a key gene regulating terpenoid biosynthesis. Therefore, FPS was overexpressed in S. baumii by Agrobacterium tumefaciens-mediated genetic transformation. The gene FPS and its downstream gene (LS) expression levels were confirmed to be increased in the FPS overexpressing transformant, and terpenoid content was 36.98% higher than that of the wild-type strain in the evaluated cultivation conditions.
桑黄是一种具有抗炎、护肝和抗肿瘤作用的药用真菌。萜类化合物是桑黄的主要药用成分之一。然而，野生型桑黄的萜类化合物产量无法满足市场需求，这影响了其在医疗保健中的应用。因此，探索如何提高桑黄中的萜类化合物含量是该研究领域的一个有前途的途径。水杨酸（SA）是一种次生代谢物。在本研究中，在真菌培养物中添加 350μmol/L 的 SA 2 天和 4 天，然后分析未经处理的菌丝体和用 SA 处理后的转录组和代谢组。萜类化合物生物合成途径中的一些基因的表达在 SA 诱导的培养物中增加，异戊烯焦磷酸（IPP）和香叶基二磷酸（GGPP）的含量显著增加，三萜类、二萜类、倍半萜类和类胡萝卜素的含量也增加。FPS 基因被认为是调节萜类化合物生物合成的关键基因。因此，通过根癌农杆菌介导的遗传转化在桑黄中过表达 FPS 基因。在 FPS 过表达转化体中，FPS 及其下游基因（LS）的表达水平被证实增加，在评估的培养条件下，萜类化合物的含量比野生型菌株高 36.98%。
