School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, China.
School of Biological Sciences, The University of Hong Kong, China.
Carbohydr Polym. 2018 Nov 15;200:144-153. doi: 10.1016/j.carbpol.2018.07.086. Epub 2018 Jul 29.
Phellinus linteus polysaccharide (PLP) has hypoglycemic effects, but mechanisms remain unclear. Male C57BL/6 J mice were either fed a normal diet (CON) or a high-fat high-fructose diet (HFD) for 16 weeks, and starting from week 12, HFD-fed animals in PLP group were orally given PLP. PLP administration significantly reduced fasting blood glucose level and ameliorated glucose intolerance. Differentially expressed genes involved in FOXO signaling pathway and in vitamin B12 (VB12) transport were identified between HFD and PLP group. HFD decreased the phosphatidylcholine (PC) to phosphatidylethanolamine (PE) ratio and S-adenosyl methionine to S-adenosyl homocysteine ratio, which were recovered by PLP treatment. Plasma VB12 levels in HFD group was lower than CON or PLP group, and PLP stimulated the proliferation of gut bacteria in genus Porphyromonas with capability of VB12 synthesis. In conclusion, PLP administration improved insulin resistance via modifying hepatic phospholipids metabolism and rescuing insulin signaling transduction.
云芝多糖（PLP）具有降血糖作用，但机制尚不清楚。雄性 C57BL/6J 小鼠连续 16 周给予正常饮食（CON）或高脂肪高果糖饮食（HFD），从第 12 周开始，PLP 组给予 HFD 喂养的动物口服 PLP。PLP 给药显著降低了空腹血糖水平，并改善了葡萄糖耐量。在 HFD 和 PLP 组之间鉴定到参与 FOXO 信号通路和维生素 B12（VB12）转运的差异表达基因。HFD 降低了磷脂酰胆碱（PC）与磷脂酰乙醇胺（PE）的比率和 S-腺苷甲硫氨酸与 S-腺苷同型半胱氨酸的比率，而 PLP 处理可恢复这些比率。HFD 组的血浆 VB12 水平低于 CON 组或 PLP 组，而 PLP 刺激了具有 VB12 合成能力的肠道细菌卟啉单胞菌属的增殖。总之，PLP 给药通过调节肝磷脂代谢和挽救胰岛素信号转导来改善胰岛素抵抗。
