Liu Zengcai, Tong Xinyu, Liu Ruipeng, Zou Li
College of Forestry, Northeast Forestry University, Harbin 150040, China.
J Fungi (Basel). 2022 Jun 21;8(7):648. doi: 10.3390/jof8070648.
Sanghuangporus baumii is a traditional medicinal fungus that produces pharmacological terpenoids, but natural resources are insufficient for applications, and its growth and development mechanisms are poorly understood. Combining metabolomic and transcriptomic analyses, we found four terpenoid hormones and a central gene, isopentenyl diphosphate isomerase (IDI), involved in growth and development. Additionally, an exogenous hormone test was used to further confirm the importance of the four terpenoid hormones. Finally, hormone content determination and qRT−PCR were performed to explore the growth and development mechanism; we found thatcis-zeatin (CZ) plays a major role in the mycelia stage, trans-zeatin (TZ) and gibberellin A4 (GA4) are important in the primordia stage, GA4 is crucial for the fruiting bodies stage, and abscisic acid (ABA) may be a marker of maturity. The IDI gene was also found to affectterpenoid hormone content by regulating the relative gene transcript levels, thereby controlling morphological changes in S. baumii. Our results revealthe growth and development mechanisms of S. baumii and may promote the breeding and utilisation of high-quality varieties.
鲍氏层孔菌是一种能产生药理萜类化合物的传统药用真菌，但自然资源不足以满足应用需求，且对其生长发育机制了解甚少。结合代谢组学和转录组学分析，我们发现了四种萜类激素和一个参与生长发育的核心基因——异戊烯基二磷酸异构酶（IDI）。此外，通过外源激素试验进一步证实了这四种萜类激素的重要性。最后，进行了激素含量测定和qRT-PCR以探究生长发育机制；我们发现，顺式玉米素（CZ）在菌丝体阶段起主要作用，反式玉米素（TZ）和赤霉素A4（GA4）在原基阶段很重要，GA4对子实体阶段至关重要，而脱落酸（ABA）可能是成熟的标志。还发现IDI基因通过调节相关基因转录水平影响萜类激素含量，从而控制鲍氏层孔菌的形态变化。我们的研究结果揭示了鲍氏层孔菌的生长发育机制，可能会促进优质品种的选育和利用。
