Department of Biology, University of Victoria, 3800 Finnerty Road, Victoria, BC, V8W 2Y2, Canada.
Golden Eagle Sablefish, 335 Walkers Hook Road, Salt Spring Island, BC, V8K 1N7, Canada.
G3 (Bethesda). 2023 Jul 5;13(7). doi: 10.1093/g3journal/jkad089.
Sablefish (Anoplopoma fimbria) are in the suborder Cottioidei, which also includes stickleback and lumpfish. This species inhabits coastal regions of the northeastern and northwestern Pacific Ocean from California to Japan. A commercial fishery for sablefish began to flourish in the 1960s, though a downward trend in stock biomass and landings has been observed since 2010. Aquaculture protocols have been developed for sablefish; eggs and sperm from wild-caught and hatchery-reared captive broodstock are used to generate offspring that reach market size in about two years. Parentage analyses show that survival in aquaculture varies among families. Growth rate and disease resistance also vary among individuals and cohorts, but the extent to which genetics and the environment contribute to this variation is unclear. The sablefish genome assembly reported here will form the foundation for SNP-based surveys designed to detect genetic markers associated with survival, growth rate, and pathogen resistance. Beyond its contribution to sablefish domestication, the sablefish genome can be a resource for the management of the wild sablefish fishery. The assembly generated in this study had a length of 653 Mbp, a scaffold N50 of 26.74 Mbp, a contig N50 of 2.57 Mbp, and contained more than 98% of the 3640 Actinopterygii core genes. We placed 620.9 Mbp (95% of the total) onto 24 chromosomes using a genetic map derived from six full-sib families and Hi-C contact data.
拟沙丁鱼（Anoplopoma fimbria）属于鳞鲀亚目，该亚目还包括棘背鱼和圆鳍鱼。该物种栖息于北太平洋东北部和西北部的沿海地区，从加利福尼亚到日本。20 世纪 60 年代，拟沙丁鱼的商业捕捞开始繁荣起来，但自 2010 年以来，其种群生物量和捕捞量呈下降趋势。已经制定了拟沙丁鱼的水产养殖方案；使用野生捕获和孵化场饲养的亲鱼的卵和精子来产生大约两年达到市场大小的后代。亲子关系分析表明，水产养殖中的存活率在不同家庭之间存在差异。增长率和抗病能力也在个体和群体之间存在差异，但遗传和环境对这种差异的贡献程度尚不清楚。本报告的拟沙丁鱼基因组组装将为基于 SNP 的调查奠定基础，旨在检测与存活率、生长速度和病原体抗性相关的遗传标记。除了对拟沙丁鱼驯化的贡献外，拟沙丁鱼基因组还可以成为管理野生拟沙丁鱼渔业的资源。本研究生成的组装体长度为 653 Mbp，支架 N50 为 26.74 Mbp，连续体 N50 为 2.57 Mbp，包含超过 3640 个硬骨鱼核心基因的 98%以上。我们使用来自六个全同胞家系和 Hi-C 接触数据的遗传图谱，将 620.9 Mbp（总长度的 95%）定位到 24 条染色体上。
