Friesen Eric R, Long Amy K, Garver Kyle A
Pacific Biological Station, Fisheries and Oceans Canada, Pacific Biological Station 3190 Hammond Bay Rd., Nanaimo, BC V9T6N7, Canada.
In Vitro Cell Dev Biol Anim. 2025 Jan;61(1):81-92. doi: 10.1007/s11626-024-00975-3. Epub 2024 Sep 30.
Sablefish Anoplopoma fimbria is a groundfish of the North Pacific Ocean typically found in sea floor habitat at depths to 2700 m. Prized as a food fish with exceptionally high market value, sablefish aquaculture has been sought to provide a sustainable source of this fish to meet market demands. While commercial culture has successfully produced market-sized fish in Pacific coastal environments, production has been hampered by disease and the overall lack of information on sablefish health and immunology. To begin to address these knowledge gaps, herein we describe the isolation and characterization of spontaneously immortalized sablefish larval cell lines (AFL). Six sublines were established from pools of early yolk-sac larvae, while attempts to develop tissue-specific-derived cell lines were unsuccessful. The six yolk-sac larval cell lines each display two morphologies in culture, an elongated fibroblast-like cell type, and a rounded squamous or epithelial-like cell type. Cytogenetic characterization suggests that both cell types are diploid (2n = 48) with 24 pairs of chromosomes, 23 pairs of autosomes, and 1 pair of sex chromosomes. A small proportion (11%) of AFL cells display tetraploidy. Incubation temperature and medium composition experiments revealed HEPES buffered L-15 media containing 10-20% FBS at temperatures between 15 and 18° C yielded optimal cell growth. These growth characteristics suggest that sablefish larval cells display a robustness for varying growth conditions. The establishment of AFL cell lines provides a foundational tool to study the physiology, health, immunology, and cell and molecular biology of sablefish.
黑鲪（Anoplopoma fimbria）是一种生活在北太平洋的底层鱼类，通常栖息于深度达2700米的海底环境中。作为一种具有极高市场价值的食用鱼，人们一直在寻求黑鲪养殖，以提供这种鱼类的可持续来源，满足市场需求。虽然商业养殖已在太平洋沿岸环境中成功培育出达到上市规格的鱼，但生产受到疾病以及黑鲪健康和免疫学方面信息普遍缺乏的阻碍。为了开始填补这些知识空白，在此我们描述了自发永生化黑鲪幼体细胞系（AFL）的分离和特性。从早期卵黄囊幼体池中建立了六个亚系，而开发组织特异性衍生细胞系的尝试未成功。这六个卵黄囊幼体细胞系在培养中均呈现两种形态，一种是细长的成纤维细胞样细胞类型，另一种是圆形的鳞状或上皮样细胞类型。细胞遗传学特征表明，这两种细胞类型均为二倍体（2n = 48），有24对染色体，其中23对为常染色体，1对为性染色体。一小部分（11%）AFL细胞呈现四倍体。孵育温度和培养基成分实验表明，在15至18°C的温度下，含有10 - 20%胎牛血清（FBS）的HEPES缓冲L - 15培养基可实现最佳细胞生长。这些生长特性表明黑鲪幼体细胞在不同生长条件下具有较强的适应性。AFL细胞系的建立为研究黑鲪的生理学、健康、免疫学以及细胞和分子生物学提供了一个基础工具。
