Bai Xinna, Gong Weifang, Guo Yaxin, Zhu Di, Li Xuemei
Collaborative Innovation Center of Tumor Marker Detection Technology, Equipment and Diagnosis-Therapy Integration in Universities of Shandong, Shandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong 276005, China.
Analyst. 2023 May 16;148(10):2327-2334. doi: 10.1039/d3an00330b.
Saxitoxin (STX) is a typical toxic guanidinium neurotoxin, one of the paralytic shellfish poisons (PSP), which poses a serious threat to human health. In this paper, a simple and sensitive SERS aptamer sensor (abbreviated as AuNP@4-NTP@SiO) for the quantitative determination of STX was developed. Hairpin aptamers of saxitoxin are modified on magnetic beads and used as recognition elements. In the presence of STX, DNA ligase, and the rolling circle template (T1), a rolling circle amplification reaction was triggered to produce long single-stranded DNA containing repetitive sequences. The sequence can be hybridized with the SERS probe to realize the rapid detection of STX. Due to the inherent merits of its components, the obtained AuNP@4-NTP@SiO SERS aptamer sensor manifests excellent sensing performance for STX detection with a wide linear range from 2.0 × 10 mol L to 5.0 × 10 mol L and a lower detection limit of 1.2 × 10 mol L. This SERS sensor can provide a strategy for the micro-detection of other biological toxins by changing the aptamer sequence.
石房蛤毒素（STX）是一种典型的有毒胍鎓神经毒素，属于麻痹性贝类毒素（PSP）之一，对人类健康构成严重威胁。本文开发了一种用于定量测定STX的简单灵敏的表面增强拉曼光谱适配体传感器（简称为AuNP@4-NTP@SiO）。石房蛤毒素的发夹适配体修饰在磁珠上并用作识别元件。在存在STX、DNA连接酶和滚环模板（T1）的情况下，触发滚环扩增反应以产生包含重复序列的长单链DNA。该序列可与表面增强拉曼光谱探针杂交以实现STX的快速检测。由于其组件的固有优点，所获得的AuNP@4-NTP@SiO表面增强拉曼光谱适配体传感器对STX检测表现出优异的传感性能，线性范围宽，为2.0×10⁻⁹ mol/L至5.0×10⁻⁹ mol/L，检测下限低至1.2×10⁻¹⁰ mol/L。这种表面增强拉曼光谱传感器可以通过改变适配体序列为其他生物毒素的微量检测提供一种策略。
