Ko Jae-Wan, Sakong Joon, Kang Sohee
Department of Dental Technology, Daegu Health College, Daegu, Korea.
Department of Public Health, Graduate School of Environment and Public Health Studies, Yeungnam University, Daegu, Korea.
J Yeungnam Med Sci. 2023 Nov;40(Suppl):S1-S8. doi: 10.12701/jyms.2023.00080. Epub 2023 Apr 26.
Residual monomer tests using high-performance liquid chromatography and cytotoxicity tests were performed to analyze the effect on the oral mucosa of a self-curing resin for provisional crown production.
A cytotoxicity test was performed to confirm whether leaked residual monomers directly affected oral mucosal cells. The cytotoxicity of the liquid and solid resin polymers was measured using a water-soluble tetrazolium (WST) test and microplate reader.
In the WST assay using a microplate reader, 73.4% of the cells survived at a concentration of 0.2% liquid resin polymer. The cytotoxicity of the liquid resin polymer was low at ≤0.2%. For the solid resins, when 100% of the eluate was used from each specimen, the average cell viability was 91.3% for the solid resin polymer and 100% for the hand-mixed self-curing resin, which is higher than the cell viability standard of 70%. The cytotoxicity of the solid resin polymer was low.
Because the polymerization process of the self-curing resin may have harmful effects on the oral mucosa during the second and third stages, the solid resin should be manufactured indirectly using a dental model.
采用高效液相色谱法进行残留单体测试，并进行细胞毒性测试，以分析一种用于制作临时冠的自凝树脂对口腔黏膜的影响。
进行细胞毒性测试，以确认泄漏的残留单体是否直接影响口腔黏膜细胞。使用水溶性四氮唑（WST）测试和酶标仪测量液体和固体树脂聚合物的细胞毒性。
在使用酶标仪的WST分析中，在液体树脂聚合物浓度为0.2%时，73.4%的细胞存活。液体树脂聚合物在浓度≤0.2%时细胞毒性较低。对于固体树脂，当使用每个样本100%的洗脱液时，固体树脂聚合物的平均细胞活力为91.3%，手工混合自凝树脂为100%，均高于70%的细胞活力标准。固体树脂聚合物的细胞毒性较低。
由于自凝树脂的聚合过程在第二和第三阶段可能对口腔黏膜产生有害影响，因此应使用牙科模型间接制作固体树脂。
