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硅酸钙基牙髓盖髓材料在人牙髓细胞中的矿化诱导潜能

Mineralization-inducing potentials of calcium silicate-based pulp capping materials in human dental pulp cells.

作者信息

Kang Sohee

机构信息

Department of Dentistry, Yeungnam University Hospital, Daegu, Korea.

出版信息

Yeungnam Univ J Med. 2020 Jul;37(3):217-225. doi: 10.12701/yujm.2020.00248. Epub 2020 May 22.

DOI:10.12701/yujm.2020.00248
PMID:32438533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7384909/
Abstract

BACKGROUND

To provide a long-term bacterial seal through the formation of reparative dentin bridge, calcium silicate-based pulp capping materials have been used at sites of pulpal exposure. The aim of this study was to evaluate the mineralization-inducing potentials of calcium silicate-based pulp capping materials (ProRoot MTA [PR], Biodentine [BD], and TheraCal LC [TC]) in human dental pulp cells (HDPCs).

METHODS

Specimens of test materials were placed in deionized water for various incubation times to measure the pH variation and the concentration of calcium released. The morphology of HDPCs cultured on the specimens was examined using a confocal laser scanning microscope (CLSM). Alizarin red S staining and alkaline phosphatase assays were used to evaluate mineralization-inducing potentials of the capping materials.

RESULTS

BD showed the highest calcium release in all test periods, followed by PR and TC. (p<0.05). All experimental groups showed high alkalinity after 1 day, except at 14 days. BD showed the highest cell viability compared with PR and TC after 1 and 3 days, while TC showed the lowest value (p<0.05). The CLSM analysis showed that cells were well adhered and expressed actin filaments for all pulp capping materials. Mineralization by PR and BD groups was higher than that by TC group based on alizarin red S staining. BD showed significantly higher alkaline phosphatase activity than PR and TC, while TC showed the lowest value (p<0.05).

CONCLUSION

Within the limitations of the in vitro study, BD had higher mineralization-inducing potential than PR and TC.

摘要

背景

为了通过形成修复性牙本质桥来提供长期的细菌封闭，基于硅酸钙的牙髓盖髓材料已被用于牙髓暴露部位。本研究的目的是评估基于硅酸钙的牙髓盖髓材料（ProRoot MTA [PR]、Biodentine [BD] 和 TheraCal LC [TC]）在人牙髓细胞（HDPCs）中的矿化诱导潜力。

方法

将测试材料标本置于去离子水中不同孵育时间，以测量pH值变化和钙释放浓度。使用共聚焦激光扫描显微镜（CLSM）检查在标本上培养的HDPCs的形态。茜素红S染色和碱性磷酸酶测定用于评估盖髓材料的矿化诱导潜力。

结果

在所有测试期间，BD的钙释放量最高，其次是PR和TC。（p<0.05）。除14天外，所有实验组在1天后均显示高碱度。在1天和3天后，与PR和TC相比，BD显示出最高的细胞活力，而TC显示出最低值（p<0.05）。CLSM分析表明，所有牙髓盖髓材料的细胞均良好粘附并表达肌动蛋白丝。基于茜素红S染色，PR和BD组的矿化程度高于TC组。BD显示出比PR和TC显著更高的碱性磷酸酶活性，而TC显示出最低值（p<0.05）。

结论

在体外研究的局限性内，BD比PR和TC具有更高的矿化诱导潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/768937277824/yujm-2020-00248f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/4719c6677797/yujm-2020-00248f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/8c840e7c7fab/yujm-2020-00248f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/7d405eda4ef8/yujm-2020-00248f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/2d4008d017f0/yujm-2020-00248f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/33f9801655a8/yujm-2020-00248f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/e683f8f5adb6/yujm-2020-00248f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/322b4ead93c6/yujm-2020-00248f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/768937277824/yujm-2020-00248f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/4719c6677797/yujm-2020-00248f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/8c840e7c7fab/yujm-2020-00248f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/7d405eda4ef8/yujm-2020-00248f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/2d4008d017f0/yujm-2020-00248f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/33f9801655a8/yujm-2020-00248f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/e683f8f5adb6/yujm-2020-00248f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/322b4ead93c6/yujm-2020-00248f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dab3/7384909/768937277824/yujm-2020-00248f8.jpg

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本文引用的文献

1
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Biomater Res. 2015 Apr 10;19:8. doi: 10.1186/s40824-015-0030-6. eCollection 2015.
2
Tomographic Evaluation of Reparative Dentin Formation after Direct Pulp Capping with Ca(OH)2, MTA, Biodentine, and Dentin Bonding System in Human Teeth.人牙用氢氧化钙、MTA、生物活性玻璃陶瓷和牙本质粘结系统直接盖髓后修复性牙本质形成的断层扫描评估
J Endod. 2015 Aug;41(8):1234-40. doi: 10.1016/j.joen.2015.03.017. Epub 2015 May 29.
3
Dentinogenic responses after direct pulp capping of miniature swine teeth with Biodentine.
牙髓盖髓材料对人牙髓干细胞成牙分化的影响：一项体外研究。
Clin Exp Dent Res. 2024 Feb;10(1):e816. doi: 10.1002/cre2.816. Epub 2023 Dec 6.
4
Ex Vivo Osteogenesis Induced by Calcium Silicate-Based Cement Extracts.硅酸钙基骨水泥提取物诱导的体外成骨
J Funct Biomater. 2023 Jun 7;14(6):314. doi: 10.3390/jfb14060314.
5
Evaluation of the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shells: an in vitro study.评估一种新型贝壳衍生生物陶瓷水泥的化学、物理和生物学性能：一项体外研究。
BMC Oral Health. 2023 Jun 3;23(1):354. doi: 10.1186/s12903-023-03073-0.
6
Cytotoxicity of dental self-curing resin for a temporary crown: an in vitro study.用于临时冠的牙科自凝树脂的细胞毒性：一项体外研究。
J Yeungnam Med Sci. 2023 Nov;40(Suppl):S1-S8. doi: 10.12701/jyms.2023.00080. Epub 2023 Apr 26.
7
Present status and future directions: Hydraulic materials for endodontic use.现状与未来方向：牙髓用液压材料。
Int Endod J. 2022 May;55 Suppl 3(Suppl 3):710-777. doi: 10.1111/iej.13709. Epub 2022 Mar 17.
用生物陶瓷对小型猪牙齿进行直接盖髓后的牙本质生成反应
J Endod. 2014 Dec;40(12):1967-71. doi: 10.1016/j.joen.2014.07.021. Epub 2014 Oct 11.
4
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J Endod. 2014 Nov;40(11):1846-54. doi: 10.1016/j.joen.2014.06.018. Epub 2014 Aug 22.
5
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6
Cytocompatibility and antibacterial properties of capping materials.封闭材料的细胞相容性和抗菌性能。
ScientificWorldJournal. 2014;2014:181945. doi: 10.1155/2014/181945. Epub 2014 May 18.
7
Hydration characteristics of Biodentine and Theracal used as pulp capping materials.用作牙髓盖髓材料的生物陶瓷和Theracal的水化特性。
Dent Mater. 2014 Jul;30(7):709-15. doi: 10.1016/j.dental.2014.03.012. Epub 2014 Apr 29.
8
Biodentine and mineral trioxide aggregate induce similar cellular responses in a fibroblast cell line.生物活性玻璃和三氧化矿物凝聚体在成纤维细胞系中诱导相似的细胞反应。
J Endod. 2014 Mar;40(3):406-11. doi: 10.1016/j.joen.2013.11.006. Epub 2014 Jan 17.
9
Characterization of set Intermediate Restorative Material, Biodentine, Bioaggregate and a prototype calcium silicate cement for use as root-end filling materials.鉴定 Set Intermediate Restorative Material、Biodentine、Bioaggregate 以及一种原型硅酸钙水泥，以用作根管充填材料。
Int Endod J. 2013 Jul;46(7):632-41. doi: 10.1111/iej.12039. Epub 2013 Jan 4.
10
The chemical properties of light- and chemical-curing composites with mineral trioxide aggregate filler.光固化和化学固化复合材料的化学性质，其中含有矿物三氧化物聚合体填料。
Dent Mater. 2013 Feb;29(2):e11-9. doi: 10.1016/j.dental.2012.11.006. Epub 2012 Nov 27.