用于研究与绝经后状态相关的特定癌症的转基因小鼠实验流程。

Sarkar Moumita, Singh Parminder, Pal Rahul

Immunoendocrinology Lab, National Institute of Immunology, New Delhi, Delhi 110067, India.

Larry L. Hillblom Center, Buck Institute for Research on Aging, Novato, CA 94945, USA.

STAR Protoc. 2023 Apr 24;4(2):102242. doi: 10.1016/j.xpro.2023.102242.

Beta-hCG is associated with poor patient prognosis in several cancers, but the particular pathophysiology of beta-hCG in post-menopausal women remains unaddressed. Specific steps to culture Lewis lung carcinoma (LLC1) tumor cells are enumerated. Ovariectomy of syngeneic, beta-hCG transgenic mice is discussed, employing a protocol designed to ensure high survival. Implantation of LLC1 tumor cells in these mice is also described. The workflow can be easily adapted in the study of other cancers associated with the post-menopausal stratum. For complete details on the use and execution of this protocol, please refer to Sarkar et al. (2022)..

β-人绒毛膜促性腺激素（β-hCG）与多种癌症患者的不良预后相关，但绝经后女性中β-hCG的具体病理生理学仍未得到解决。文中列举了培养Lewis肺癌（LLC1）肿瘤细胞的具体步骤。讨论了对同基因β-hCG转基因小鼠进行卵巢切除术，采用了旨在确保高存活率的方案。还描述了在这些小鼠中植入LLC1肿瘤细胞的过程。该工作流程可轻松应用于与绝经后阶层相关的其他癌症研究。有关本方案使用和实施的完整详细信息，请参阅Sarkar等人（2022年）的文章。